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Title:: Research of “Minimal Wound Brush” to Acquire Genetically Modified Glyphosate-resistant Soybean

Author(s):: RONG Fei¹; WANG Gang¹; JI Jing¹; WANG Ping²; DONG Xin²; GAO Hai-ling¹; CAO Yue-ping³; QIU Li-juan⁴; 1School of Environmental Science and Engineering, Tianjin University, Tianjin 300072,China;?
2School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005,China;
3School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200436,China;?
4Chinese Academy of Agricultural Sciences, Beijing 100081,China

Keywords:: Soybeans; Embryonic tips apical point; “Minimal wound brush”; EPSPS gene

PACS:: S565.1

DOI:: 10.11861/j.issn.1000-9841.2015.02.0217

Abstract:: Growing points of embryonic tips from mature soybeans after 3 days post germination were used as a receiving point of Agrobacteria carrying recombinated vectors for the transformation of glyphosate-resistant gene (EPSPS) into soybeans (Suinong 22) by using “minimal wound brush” method, and transformed T₁?plants were screened by glyphosate and tested by PCR. Chlorophyll content index, shikimic acid concentration, and photosynthetic rate of wild-type and transgenic soybean plants were determined.The results showed that surviving rate of T₀ soybean plants was 97.38%, transformation rate for T₁?soybean plants was 6.20%.Meansurements of related physiological factors from wild-type and transgenic plants were taken and it was found that chlorophyll content index and photosynthetic rate were higher in trangenic plants than wild types; meanwhile, shikimic acid concentration were much lower in transgenic plants than wild types.The above physicological results confirmed the resistance of glyphosate in transgenic soybeans plants.

References:

［1］钟金传, 吴文良, 夏友富.转基因大豆发展及中国大豆产业对策［J］中国农业大学学报, 2005, 10(4): 43-50(Zhong J Z,Wu W L, Xia Y F, et al.Development of genetically modified soybean and strategy for soybean industry of China［J］Journal of China Agricultural University, 2005, 10(4): 43-50)

［2］邱丽娟, 王昌陵, 周国安, 等.大豆分子育种研究进展［J］中国农业科学, 2007, 40(11): 2418-2436(Qiu L J, Wang C L, Zhou G A, et al.Soybean molecular breeding［J］ Scientia Agricultura Sinica, 2007, 40(11): 2418-2436)

［3］杨桂英,马绍宾,何瀚.大豆的遗传特点、品质改良与育种难点［J］贵州农业科学,2002, 30(6): 57-60(Yang G Y, Ma S B, He H.Genetic characters, quality amelioration and difficulties in breeding of soya ［J］Guizhou Agricultural Sciences,2002, 30(6):57-60)

［4］Cahoon E B.Genetic enhancement of soybean oil for industrial uses: Prospects and challenges［J］Ag Bio Forum, 2003, 6(1-2 ):11-13

［5］杜艳艳.全球转基因作物商业化发展趋势［J］浙江农业科学,2010(6): 1177-1181（Du Y Y.Trends of global commercial development of GM crops［J］Journal of Zhejiang Agricultural Sciences, 2010(6): 1177-1181)

［6］苏少泉.草甘膦述评［J］. 农药, 2005, 44(4): 145-149(SU S Q. Glyphpsate review［J］Chinese Journal of Pesticides, 2005, 44(4): 145-149)

［7］余文哲,杜艳艳.美国主要转基因作物发展现状及启示［J］中国生物工程杂志, 2012, 32(1): 129-133(Yu W Z, Du Y Y.Status and enlightenment of major genetically engineered crops in the U.S［J］China Biotechnology,2012,32(1):129-133)

［8］叶汉英,杨伟华.转基因大豆的发展及其安全性评价［J］粮油加工, 2007(4): 45-48（Ye H Y，Yang W H. The development and safety evaluation of transgenic soybean［J］Cereals and Oils Processing，2007(4): 45-48）

［9］叶增民, 潘婕.转基因大豆及其制品的安全性研究现状［J］生物技术通报, 2009(2): 26-28（Ye Z M, Pan J. Current situation in safety of transgenic soybean and its products［J］ Biotechnology Bulletin，2009(2): 26-28）

［10］Owen M D K.Current use of transgenic herbicide-resistant soybean and corn in the USA［J］XIVth International Plant Protection Congress,1999,7:25-30

［11］潘良文,陈家华，沈禹飞，等.进口转基因抗草甘膦油菜籽和大豆中CP4-EPSPS基因的检测比较研究［J］生物技术通迅,2001 ,21(3):175-177,207（Pan L W，Chen J H, Shen Y F, et al. Detection comparison of CP4-EPSPS gene from genetically modified Roundup-Ready rapeseed and soybean［J］Letters in Biotechnology，2001,21 (3):175-177,207）［12］向文胜.抗除草剂草甘膦转基因作物［J］东北农业大学学报,1998,29(1):92-98(Xiang W S.Glyphpsate resistant transgenic crops［J］Journal of Northeast Agricutureal University, 1998,29(1):92-98)

［13］de Block M, Herrera.Estrella L, van Montagu M, et al. Expression of foreign genes in regenerated plants and their progeny［J］The EMBO Journal,1984, 3: 1681-1689

［14］Horseh R B, Fraley R T, Rogers S G. Inheritance of functional foreign genes in plants［J］ Science, 1984, 223:496-498

［15］王振华,杨德光,张辉,等.大豆遗传转化技术在转基因大豆研究中的应用［J］生物技术通报,2010（10）:60-66（Wang Z H, Yang D G, Zhang H, et al.Research progress of transgene method in transgenic soybean (Glycine max L)［J］Biotechnology Bulletin,2010(10):60-66)

［16］Zhang Z, Guo Z, Shou H, et al.Assessment of conditions affecting Agrobacterium-mediated soybean transformation and routine recovery of transgenic soybean［J］ P1ant Genetic Engineering, 2000(5): 88-94

［17］王萍，张淑珍，李文滨，等.大豆不同基因型胚尖不定芽的诱导及对抗生素的敏感性［J］作物杂志，2010(2)：50-53(Wang P, Zhang S Z, Li W B, et al.Induction of adventitious shoots from embryonic tip of diferent soybean gentotypes and their sensibility to antibiotics［J］Crop,2010(2)：50-53)

［18］王罡,季静,郑丽红,等.口杯法便捷外植体灭菌的方法［P］中国:201110202739-7 (Wang G, Ji J, Zheng L H, et al.A convenient method tosterilize explants use cups［P］China： 201110202739-7

［19］张艳敏, 杨帆, 游颜杰, 等. 小麦茎生长点转化研究初报［J］华北农学报, 2005, 20(1): 17-22(Zhang Y M,Yang F, You Y J, et al.In planta transformation of wheat apical meristem：A preliminary study［J］Acta Agriculturae Boreali-sinica,2005, 20(1): 17-22)

［20］郭华, 罗军武, 周建平, 等.几种油料的子叶细胞形态与主要化学成分分析［J］现代食品科技, 2006, 22(4): 33-36(Guo H, Luo J W, Zhou J P, et al.The morphology of cotyledon cells and chemical components of some oil raw stuff［J］Modern Food Science and Technology, 2006, 22(4): 33-36)

［21］原向阳, 郭平毅, 张丽光, 等.不同时期喷施草甘膦对大豆生理指标的影响［J］中山大学学报(自然科学版), 2009, 48(2): 90-94(Yuan X Y, Guo P Y, Zhang L G, et al. Impact of spraying glyphosate on physiologi physiological index of soybean at diferent growth stages［J］Journal of Sun Yat.sen University(Natural Science),2009, 48(2): 90-94)

［22］陈虎保, 陈国海.草甘膦的作用机理及部位［J］林业科技通讯, 1997 (1): 23-25(Chen H B，Chen G H.Mechanism and site of action of glyphosate［J］Forest Science and Technology, 1997 (1): 23-25）

［23］原向阳，郭平毅，张丽光, 等.第三复叶期喷施草甘膦对转基因大豆和普通大豆生理指标的影响［J］中国农业科学, 2008, 41(11): 3886-3892( Yuan X Y, Guo P Y, Zhang L G, et al. Impact of glyphosate on physiological index of transgenic and conventional soybean on three-trifoliolate leaf stage［J］Scientia Agricultura Sinica,2008, 41(11): 3886-3892)

Research of “Minimal Wound Brush” to Acquire Genetically Modified Glyphosate-resistant Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

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