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Isolation and Bioinformatics Analysis of GmSbh1 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年02期
Page:
212-216,223
Research Field:
Publishing date:

Info

Title:
Isolation and Bioinformatics Analysis of GmSbh1 in Soybean
Author(s):
SHU Ying-jie1; 2;  TAO Yuan1;  WANG Shuang1;  HUANG Li-yan1;  CHEN Ming1;  MA Hao1
1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China；?
2College of Agriculture, Anhui Science & Technology University, Fengyang 233100, China
Keywords:
Soybean; Homeobox gene; Gene isolation; Bioinformatics analysis
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.02.0212
Abstract:
The cDNA sequences of GmSbh1-gene in soybean were isolated, and its bioinformatics character was analyzed. The results showed that there was an intact ORF in 156-1 293 bp , and it encoded a 42.37 kDa protein with 379 amino acids.The GmSBH1 protein contains the KNOX1, KNOX2, ELK and Homeodomain (HD).There were 18 homologous sequences that have more than 80% identity with GmSbh1 GmSBH1 was a hydrophilic protein without signal peptide and contained 10% Ser, 9.8% Asn and 8.4% Leu, the phosphorylation sites were located at Ser(13), Tyr(2) and Thr(1), respectively.Secondary structure prediction results showed that the GmSBH1 protein contains alpha-helix(39.31%), beta turn (5.28%), extended strand(8.44%) and random coil (46.97%), but without transmembrane helical structure. The probability of GmSH1 interacted with RPL, BEL1, AT4G32980.1-P, BLH2 and BLH3 was higher, and coexpression with BEL1 in .Arabibidopsis.

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Last Update: 2015-06-07