|Table of Contents|

Optimization of Immature Seed Cotyledon-node Transformation System and Development of Genetically Modified Soybean by Transformation of EPSPS and GAT genes(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年02期
Page:
199-204
Research Field:
Publishing date:

Info

Title:
Optimization of Immature Seed Cotyledon-node Transformation System and Development of Genetically Modified Soybean by Transformation of EPSPS and GAT genes
Author(s):
ZHANG Xian-li12GUO Yong2JIN Long-guo2ZHANG Li-juan2QIU Li-juan2
1College of Agriculture, Northeast Agricultural University, Harbin 150030, China;?
2The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) / MOA Key Lab of Soybean Biology (Beijing), Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Keywords:
Soybean Immature seed Cotyledonary node Transformation
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.02.0199
Abstract:
Simple and efficient genetic transformation systems are very important for breeding transgenic soybeans and performing functional genomics study. In order to develop a concise and highly efficient Agrobacterium-mediated transformation system in soybean, transformation methods using immature seeds and mature seeds were compared.The effect of different factors on the .Agrobacterium-mediated transformation of immature seeds was investigated using the rate of resistant bud formation. These factors affecting transformation include selection pressure and the physiological state of the immature seeds Finally, the Agrobacterium-mediated transformation system of immature seed was optimized. Nine transgenic plants were screened with 15 mg?L-1?glyphosate and they grew normally and fertilityPCRsequencing and Southern Blot analysis also showed that the foreign genes had been integrated into the genome of the T0?individuals PCR analysis of 2 lines from the T1?generation also showed that the separation ratio fit 3∶1 The glyphosate tolerant soybeans obtained in this study by phenotype identification provide new materials for breeding of transgenic soybean with glyphosate tolerance.

References:

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Last Update: 2015-06-07