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Research on Liquid Fermentation Process of Nattokinase(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年01期
Page:
128-130
Research Field:
Publishing date:

Info

Title:
Research on Liquid Fermentation Process of Nattokinase
Author(s):
REN Wu-xian; WANG Xing-xing; DAI Dong-sheng
Yabao Pharmaceutical Group Co. Ltd, Ruicheng 044600,China
Keywords:
Nattokinases; Fermentation; Carbon source; Dissolved oxygen
PACS:
TQ920.6
DOI:
10.11861/j.issn.1000-9841.2015.01.0128
Abstract:
The liquid fermentation process of nattokinase was studied both in shake flask experiment and 5L fermentor. The results showed that the best condition was 4% (w/v) soluble starch as carbon source, 10 h bacteria age, 3% (v/v) inoculum concentration, 0.5 vvm ventilation，and the rotate speed of fermentor increased from 220 r?min-1?to 700 r?min-1. All of those above could keep the dissolved oxygen in fermentor above 20%. After optimized, the expression quantity of Nattokinase in the shaker increased from lower than 1 000 IU?mL-1?to 2 051.3 IU?mL-1, equal to urokinase.

References:

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Last Update: 2015-04-13