|Table of Contents|

WU Yan-yan, DAI De-yan, CAI Chun-mei(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年01期
Page:
112-115
Research Field:
Publishing date:

Info

Title:
WU Yan-yan, DAI De-yan, CAI Chun-mei
Author(s):
College of Life Science/Key Lab of Plant Biotechnology in Universities of Shandong;  Qingdao Agricultural University;  Qingdao 266109;  China
mUREA; Soybean; Genomic DNA extraction
Keywords:
It is difficult to extract high quality and sufficient yield of genomic DNA for soybean molecular biological research;  because of a great deal of protein;  polysaccharides;  hydroxybenzenes and esters. A modified UREA (mUREA) protocol for extracting soybean DNA was provided in this paper. We precipitated DNA with isopropanol and washed DNA pellet with washing buffer. Using soybean leaves and seeds as material;  compared mUREA method with CTAB and SDS methods. The results showed that the quality and yield of DNA from soybean leaves were highly significant than using mUREA method. And using soybean seeds as material;  the purity of DNA from mUREA was better than the other two methods. So mUREA method yielded much more DNA of high quality for soybean leaves and seeds that was suitable for various molecular operations.
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.01.0112
Abstract:
A Modified UREA Protocol for Soybean DNA Extraction

References:

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Last Update: 2015-04-13