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Effects of AMP on the Al-induced Citrate Exudation and Al-resistance of Tamba Black Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年01期
Page:
82-86
Research Field:
Publishing date:

Info

Title:
Effects of AMP on the Al-induced Citrate Exudation and Al-resistance of Tamba Black Soybean
Author(s):
GUO Chuan-long;  ZHAO Yan;  WU Kong-huan;  LI Kun-zhi;  CHEN Li-mei
Biotechnology Research Centre, Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Kunming 650500, China
Keywords:
Al stress;  Al resistant black soybean;  14-3-3 protein; Plasma membrane H+-ATPase; Adenosine 5’-monophosphate
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.01.0082
Abstract:
To investigate the effect of Adenosine 5’ monophosphate (AMP) on the citrate secretion and Alresistance of Alresistant Tamba black soybean (RB). RB was cotreated with AMP and 50 mmol?L-1?Al. The results showed that the relate root growth rate of RB cotreated with 100 mmol?L-1?AMP and 50 mmol?L-1?Al for 8 h was significantly lower than that of the plants treated with 50 mmol?L-1?Al alone. Co-immunoprecipitation showed that the presence of AMP under Al stress decreased the Al-enhanced interaction of PM H+-ATPase and 14-3-3 protein in root tips of RB, which reduced PM H+-ATPase activity by approximately 1-fold and thereby led to a significant decrease in H+ efflux and citrate secretion (approximately 1-fold) in RB roots. Those results indicated that AMP decreases H+ activity and citrate exudation in RB via its inhibition on the interaction between 14-3-3 protein and PM H+-ATPase under Al stress, thereby reducing the relate root growth rate of RB and its Al-tolerance.

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Last Update: 2015-04-12