|Table of Contents|

Screening of Appropriate PEG-6000 Concentration for the Identification of Soybean Drought Tolerance at Germination Stage(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年01期
Page:
56-59
Research Field:
Publishing date:

Info

Title:
Screening of Appropriate PEG-6000 Concentration for the Identification of Soybean Drought Tolerance at Germination Stage
Author(s):
SHU Ying-jie;  ZHOU Yu-li; SHI Xia-qing; HU Neng-bing; SHAO Qing-qin; DU Jun-li
Agricultural College of Anhui Science and Technology University, Fengyang 233100, China
Keywords:
Soybean(Glycine max);  Polyethylene glycol (PEG-6000);  Drought stress; Germination stage; Drought tolerance
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.01.0056
Abstract:
Three soybean varieties（Ningzhen 1，Lee68 and Xiangdou 3）were used to study the effect of different PEG-6000 concentrations (5%, 10%, 15%, 20%, 25% and 30%)on germination of soybean seed, membrane lipid peroxidation and osmotic substances of soybean seedlings. Results showed that when the PEG-6000 concentration less than 10%，which can promote soybean seeds germination, improve the germination potential and germination rate significantly. When the PEG-6000 concentrations were more than 25%, with the increasement of PEG-6000 concentration,germination potential, germination rate, germination index and simple vigor index of soybean seed decreased significantly.The contents of malondialdehyde(MDA), proline (Pro), soluble protein and soluble sugar increased significantly with the PEG-6000 concentration increased, especially when the PEG-6000 concentration was between 15% and 25%.We can conclude preliminarily that the appropriate PEG-6000 concentration for the identification of soybean drought tolerance in seed germination stage was 20%-25% by synthesizing seed germination, membrane lipid peroxidation and the content change of osmotic substances.

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Last Update: 2015-04-12