|Table of Contents|

Construction of Bivalent Plant Expression Vector with Two Broad-Spectrum Disease Resistance Genes chi and hrpZpsta and Transformation into Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年01期
Page:
26-31
Research Field:
Publishing date:

Info

Title:
Construction of Bivalent Plant Expression Vector with Two Broad-Spectrum Disease Resistance Genes chi and hrpZpsta and Transformation into Soybean
Author(s):
LU Shi;  WANG Pi-wu;  QU Jing;  MA Jian;  ZHANG Zhuo;  WU Nan;  CAI Yuan
Agronomy College of Jilin Agricultural University, Changchun 130118, China
Keywords:
Soybean;  chi-hrpZpsta bivalent vector; Southern blot; Q-PCR
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.01.0026
Abstract:
Soybean fungous disease has become the main cause that limited soybean yield increasing and quality enhancement, one of the effective ways to enhance the resistance of soybean is using genetic engineering technology to cultivate soybean varieties with broad-spectrum disease resistance. In this research, two broad-spectrum disease resistance genes were built into bivalent plant expression vector, imported into soybeans by Agrobacterium-mediated method, and obtained transgenic soybeans with gene expression at the transcriptional level. After PCR, southern blot analysis and Q-PCR detection on resistant positive plants and their offspring, 7 T0 positive plants and 27 T1 positive plants were obtained. Different situation occurred when exogenous gene integrated with transformation of plant genome,chi-hrpZpsta bivalent expression vector in the form of monovalent and bivalent random integrated into the recipient soybean genome and could be genetic stabilized, meanwhile at the transcriptional level were different as well.

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Last Update: 2015-04-10