|Table of Contents|

Cloning and Expression Pattern Analysis of a RNA-dependant RNA Polymerase Gene GmRDR1 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年01期
Page:
19-25
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Pattern Analysis of a RNA-dependant RNA Polymerase Gene GmRDR1 in Soybean
Author(s):
WU Bing-yue1; 2;  SHEN Liang1; 2; SONG Pu-wen1; 3;  CHEN Hua-tao1;  CUI Jin2;  XU Xiao-ming2; CHEN Xin1
1.Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2.College of Life Science, Nanjing Agricultural University, Nanjing 210095, China;
?3.College of Agronomy, Nanjing Agricultural University, Nanjing 210095, China
Keywords:
Soybean;  Gene cloning;  Expression pattern;  Biotic stress;  Abiotic stress;  Subcellular localization
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.01.0019
Abstract:
In order to find new resistant gene and cultivate new resistant varieties of soybean, a novel soybean gene named GmRDR was identified using homologous cloning method. Sequence of the gene was analyzed by bioinformatics software, expression pattern of the gene in different tissues and its response to biotic and abiotic stresses were performed using real-time PCR. The transformation system of onion epidermal cells mediated Agrobacterium was established for subcelluar localization. The results showed that GmRDR1 gene was located on chromosome 2 in soybean and the length was 3 956 bp. The ORF of GmRDR1 was 3 378 bp and encoded 1 125 amino acids(279.72 kDa) with an isoelectric point of 4.63. The consereved motif ‘DLDGD’ of RDRs gene family was identified in GmRDR1. The expression pattern of the gene was identified in all tissues of soybean, and showed the highest expression level in leaves of soybean; Real-time PCR analysis revealed that mRNA levels of the gene in the resistant material Kefeng 1 were much higher than the susceptible material Nannong 1138-2 after soybean mosaic virus(SMV) treatment. For abiotic stress, GmRDR1 showed high expression levels under salt stress and drought treatment. There was an early response after 6 hours ABA treatment, the expression level of GmRDR1 gene showed a suddenly rise after 24 hours under chilling stress. The result of subcellular localization revealed that GmRDR1 gene encoding the protein was located in the nucleus. Therefore, a preliminary judgment was that GmRDR1 gene had the function of salt and drought tolerance, and it was involved in the resistant response in soybean. The new gene showed the value for breeding resistant soybean cultivar by molecular breeding method.

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Last Update: 2015-04-09