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Screening and Identification of Three Types of Soybean Lines Lacking Different Seed Storage Protein Subunits(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2015年01期
Page:
1-8,31
Research Field:
Publishing date:

Info

Title:
Screening and Identification of Three Types of Soybean Lines Lacking Different Seed Storage Protein Subunits
Author(s):
ZHANG Guo-min1; ZHANG Ya-qin1; SHU Ying-jie2; MA Hao1
1State Key Laboratory of Crop Genetics & Germplasm Enhancement/Department of Seed Industry Science, Nanjing Agricultural University, Nanjing 210095, China；
2Agricultural College,Anhui Science and Technology University,Fengyang 233100,China
Keywords:
Soybean (Glycine maxL.Merr.) ;  Storage protein;  Subunit deficiency;  Germplasm enhancement;  Evalution
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2015.01.0001
Abstract:
Improvement of protein components is one of the hotspots in the protein quality breeding of soybean. Soybean seed storage proteins mainly consist of 11S globulin and 7Sβ-conglycinin. They determine the nutritional value and functional properties of soy proteins. In the present study, natural mutants of subunit deficiency were used through pyramiding breeding to produce new deficiency types. After screening with polyacrylamide gel electrophoresis (SDS-PAGE), lines lacking A3+α′subunits, lines only containing βsubunit, lines only containing α+βsubunits, lines only containing α′+βsubunits, lines only containing 7S subunits, lines lacking A5A4B3 subunits, lines with the lower level of A2A1aA1b?subunit and lines with the lower level of B1aB1bB2 subunit, etc, were obtained. The lines lacking A3+α′ subunits, containing only α′+β subunits, and containing 7S subunits could normally grow and develop as well as were stably inherited. The lines containing only 7S subunits had the highest amount of total 7S while the lines lacking A3+α’ subunits possessed the highest level of 11S. The variation range for the 11S/7S ratio in the three new types was between 0.14 and 1.27, while their growth period was between 105 and 111 d. All the three new types had almost the same hundred-grain weight. The lines lack of A3+α′ subunits had the highest yield per plant of 55.12 g. The lines containing only α+β subunits had the lowest amount of protein and oil (57.6%). The order of protein contents of the lines were as follows: lines containing 7S subunits>lines lacking A3+α’ subunits>lines containing only α+βsubunits. The order of fat content was as follows: lines containing only α+βsubunits>lines containing only 7S subunits>lines lacking A3+α’ subunits.

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Last Update: 2015-04-09