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Screening and Optimization of the Culture Conditions for Soybean Rhizobium Strain AHM2B(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年06期
Page:
953-956
Research Field:
Publishing date:

Info

Title:
Screening and Optimization of the Culture Conditions for Soybean Rhizobium Strain AHM2B
Author(s):
WU Ping; LI Zheng-peng; HE Qing-yuan; SHI Jun; ZHU Chang-wei
Institute for Applied Microbiology,Anhui Science and Technology University,Fengyang 233100，China
Keywords:
Soybean rhizobia; Medium; Optimization of the culture conditions; Orthogonal experiment
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.06.0953
Abstract:
The growth conditions of the Soybean Rhizobium strain AHM2B were compared in the YMA,TY,PA and BSE mediums.The results showed that the strain grew faster in the BSE medium than in the others.The single factor experiments showed that the best carbon and nitrogen sources was sucrose and yeast extract,respectively,pH was 8.0,culture at 28℃,and 4% inoculation volumes.The best culture conditions were obtained by orthogonal test,the results were:sucrose 10 g,yeast extract 3 g,MgSO4.H2O 0.2 g,K2HPO4?0.5 g，NaCl 0.1g，CaCl2.H2O 0.1g，Rh solution 4 mL，bean spouts extract 1 000 mL，pH7.0,inoculation volumes 4% and culture at 28℃.

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Last Update: 2014-12-29