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Screening and Characterization of Superior Nitrogen-Tolerance Soybean Rhizobia(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年06期
Page:
861-865
Research Field:
Publishing date:

Info

Title:
Screening and Characterization of Superior Nitrogen-Tolerance Soybean Rhizobia
Author(s):
BAI YuGUAN Da-weiLI LiJIANG XinMA Ming-chaoLI Jun
Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences/Laboratory of Quality & Safety Risk Assessment for Microbial Products,Ministry of Agriculture,Beijing 10081,China
Keywords:
ScreeningSoybean rhizobiaNitrogen-toleranceSuperior strain
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.06.0861
Abstract:
The superior nitrogen-tolerance rhizobia strains were screened from 254 strains of rhizobia,which were isolated from 7 different fertilization treatments in long-term black soil fertility and fertilizer efficiency experiment station adopting high-concentration nitrogen plate method and vermiculite pot.The black soil fertility and fertilizer efficiency experiment station were constructed by Heilongjiang Academy of Agricultural Sciences in 1979.The results obtained from high-concentration nitrogen plate method indicated that increasing urea concentration had an increased bacteriostatic effect.Urea concentration which could seriously inhibit growth of strains was 5 g.L-1,only 11 strains could grow in this urea concentration,which were all isolated from continuous application of nitrogen fertilizer.In the subsequent high-concentration nitrogen vermiculite pot experiment,the best strain 5841 were selected according to the plant dry weight,number of nodules,dry weight of nodules and total N content of the plant,which had the ability of nodulation nitrogen fixation in high-concentration nitrogen.Based on 16S rDNA sequence phylogenetic analysis,the strain 5841 was identified as Brandyrhizobium japonicum.

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Last Update: 2014-12-29