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Genomic Library Construction of Bacillus Megaterium Resistant to 2,4-D(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年06期
Page:
826-829
Research Field:
Publishing date:

Info

Title:
Genomic Library Construction of Bacillus Megaterium Resistant to 2,4-D
Author(s):
XU Si-liang12HAN Chao12JIN Long-guo2QIU Li-juan2TAO Bo1
1.College of Agriculture,Northeast Agricultural University,Harbin 150030,China;
2.The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Germplasm & Biotechnology(MOA),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China
Keywords:
24-D resistantBacillus megateriumGenomic libraryTransgenic soybean
PACS:
Q933
DOI:
10.11861/j.issn.1000-9841.2014.06.0826
Abstract:
Bacillus megateriumwhich was efficient resistant to 2,4-D,recycled the 1~4 kb DNA fragments of digested B.megaterium genomic DNA,and with digested vector pACYC184 overnight connection,transferred it to BL21 competent cells,after the blue-white screening and chloramphenicol resistance screening by colony PCR to detect the connection efficiency and quality of the library.Successfully constructed the B.megaterium genomic library and obtain 8 900 positive clones with an average insert fragment length of 2.5 kb.And this library covers approximately 556 times B.megaterium genome.This library establish the theoretical basis for 2,4-D resistant transgenic soybean.

References:

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Last Update: 2014-12-29