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Cloning,Plant Expression Vector Construction and Preliminary Study of GmSOT1 Gene from Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年06期
Page:
815-819
Research Field:
Publishing date:

Info

Title:
Cloning,Plant Expression Vector Construction and Preliminary Study of GmSOT1 Gene from Soybean
Author(s):
XIE Lu-yao1; 2; LI Xiao-lin2; LUO Li3; WEN Tao1; ZHU Mu-lan2
1.Agricultural College,Sichuan Agricultural University,Chengdu 611130,China;
2.Institute of Plant Physiology & Ecology,Shanghai Institutes for Biological Sciences,CAS,National Center for Plant Gene Research(Shanghai),Shanghai 200032,China;
3.Shanhai Key Lab of Bioenergy Plant,School of Life Science,Shanghai University,Shanghai 200444,China
Keywords:
Soybean; Sulfotransferase(SOT); Glucosinolate; Gene cloning; Vector construction
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.06.0815
Abstract:
Sulfotransferase(SOT)is one of the most important key enzymes involved in the biosynthesis of the core structure of glucosinolate.Here GmSOT1 was cloned from the soybean genome using reverse genetics to investigate its preliminary functions.An ORF(open reading frame)DNA of GmSOT1 was amplified by PCR and ligated into a pRNAi vector under control of the 35S(CaMV35S)promoter.The 35S:GmSOT1 DNA fragment was excised from the pRNAi vector and ligated into the multiple cloning sites of pCAMBIA2301,named as pCAMBIA2301-35S:GmSOT1.The 35S-GmSOT1 construct was introduced into mature embryo cells of soybean through Agrobacterium-mediated genetic transformation.The transformed soybean embryos were used for preliminary phenotype analysis.Our results showed that the cloned GmSOT1 gene(CDS)-consisted of 1 014 bp nucleotide acids and encoded a 337 amino-acid protein.The construct of 35S:GmSOT1 was successfully introduced into soybean embryo cells.The transformed radicles appear enlarged and curved.The results provide a new cue to further study on the function of GmSOT1.

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Last Update: 2014-12-25