|Table of Contents|

Molecular Characterization of the Transgenic Soybean Plants by Sonication-assisted Agrobacterium-mediated Transformation Using the Embryonic Tips(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年06期
Page:
808-814
Research Field:
Publishing date:

Info

Title:
Molecular Characterization of the Transgenic Soybean Plants by Sonication-assisted Agrobacterium-mediated Transformation Using the Embryonic Tips
Author(s):
SUN Shi-jing1; YANG Su-xin1; 2; FENG Xian-zhong1; 2
1.College of Life Science,Shandong Normal University/Key Laboratory of Systems Biology in Universities of Shandong,Jinan 250014,China;
2.Key Laboratory of Soybean Molecular Design Breeding,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Changchun 130102,China
Keywords:
Glycine max; Embryonic tips; Agrobacterium-mediated transformation; Sonication-assisted treatment
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.06.0808
Abstract:
Agrobacterium-mediated transformation as a practical and common method for introducing specific DNA fragments into plant genomes is well established and the number of transgenic plants produced using this method is increasing.Despite the popularity of the method,low efficiency of soybean transformation is a major challenge for scientists.Modification of transformation method may lead to better understanding of the system and result in high efficiency transformation.In this paper,the sonication-assisted Agrobacterium-mediated transformation(SAAT)system of soybean(Glycine max)was set up using embryonic tips of soybean cultivar of Williams 82.The effects of different SAAT duration and glufosinate concentration on transformation efficiency were investigted.The results showed that the transient expression of UidA(GUS)reporter gene reached the hightest with 2 minutes sonication treament among various time durations.The gradient seletion of glufosinate concentration from 0.3 to 0.6 mg.L-1produced the highest transformation rate.Transgenic plants were verified by PCR analysis and herbicide paint assay.Real-Time Quantitative PCR showed the expression of target genes in transgenic plant was about 1.7 to 42 times than that in wild type.Southern blot result proved the target gene was integrated into the soybean genome of transgenic plants.This research will provide new information for soybean transformation improvement.

References:

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Last Update: 2014-12-25