|Table of Contents|

Genetic Diversity of Sclerotinia Sclerotiorum in Soybean in Different Mycelia Compatibility Groups(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年05期
Page:
718-723
Research Field:
Publishing date:

Info

Title:
Genetic Diversity of Sclerotinia Sclerotiorum in Soybean in Different Mycelia Compatibility Groups
Author(s):
LI Yi-chu1; 2; ZHANG Jun-hua1
1.Agronomy College，Northeast Agricultural University,Harbin 150030,China;
2.Plant Protection Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，China
Keywords:
Soybean Sclerotinia sclerotiorum; Mycelia compatibility group; Morphology; Genetic diversity
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0718
Abstract:
To identify genetic diversity of isolates of Sclerotinia sclerotiorum- from different mycelia compatibility groups,12 alone representative strains of different MCGs were selected by the Mycelia compatibility groups(MCG)screening methods.The strains were cultivated by PDA medium,and the colony characteristics,growth rate of mycelium and morphological features of sclerotia were compared.Genetic polymorphism was studied by SSR molecular marker.The results showed that the differences were significant in colony characteristics,mycelium growth rate,Sclerotia location on medium,the total number of Sclerotia and Sclerotia weight among the strains from different MCGs.The colony shape,colony color and hyphal fullness degree of strains were divided into two categories.Sclerotium diameter changed from 0.13 to 0.53 cm,Sclerotium grain weight ranged from 0.007 to 0.340 g.The average growth rate of strain was 2.206 cmd-1,the capability sclerotial formation was not affected by mycelium growth rate.The result of SSR molecular marker technology was similar with MCG’s,and the higher of the similarity coefficient,the higher consistence.Therefore,there are scores of mycelia compatibility groups of S.sclerotiorum in soybean in Heilongjiang province,the strains of S.sclerotiorum had polyphyly and diversity.It indicated the presence of heterogeneity and heredity types diversity of S.Sclerotiorum infecting soybean in Heilongjiang province.

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Last Update: 2014-12-27