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Comparison on Adventitious Buds Induction Capacities of Two Kinds of Explants in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年05期
Page:
680-684
Research Field:
Publishing date:

Info

Title:
Comparison on Adventitious Buds Induction Capacities of Two Kinds of Explants in Soybean
Author(s):
WANG Gang1WANG Jiao23WANG Ping2JI Jing1
1.School of Environmental Science and Engineering,Tianjin University,Tianjin 300072,China;
2.School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang 222005,China;
3.School of Life Science,Jiangsu Normal University,Xuzhou 221116,China
Keywords:
Soybean(Glycine max L.)Embryonic tipHypocotylAdventitious bud
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0680
Abstract:
To compare regeneration capacities of two kinds of explants in soybean (Glycine max L.),the induction rate,number and length of adventitious buds were investigated with embryonic tips and hypocotyls as explants.The results showed that the adventitious bud production depended on the genotypes applied in soybean.In the embryonic tip regeneration system,the maximum induction rate of adventitious buds was observed in Jilin 35,and the maximum number of adventitious buds was observed in Jinlin 35 and Hefeng 35,while as the longest adventitious buds appeared in Heinong 44.In the hypocotyls regeneration system,desired induction rates of adventitious buds were realized in Huaidou 4,Heinong 44,Huaidou 9 and Jinlin 35.Similar number of adventitious buds were achieved in all of those genotypes except for Heinong 40 possessing less adventitious buds.Furthermore,the maximum length of adventitious buds appeared in genotypes Huaidou 9,Heinong 44 and Hefeng 35.The results indicated that Hefeng 35 had the best regeneration capacity in both regeneration systems,based on the data from induction rates of adventitious buds,number and length of adventitious buds,and their regeneration capacities.In addition,Heinong 40 may to be more suitable for embryo tip regeneration system,and Huaidou 9 was more desirable for hypocotyl regeneration system.

References:

[1]王萍,王罡,吴颖,等.大豆组织培养的研究进展[J].大豆科学,2003,22(2):142-145.(Wang P,Wang G,Wu Y,et al.Current progress on tissue culture of soybean[J].Soybean Science,2003,22(2):142-145.)

[2]李明春,蔡易,赵桂兰,等.改良大豆子叶节再生体系的研究[J].作物学报,2006,32(2):223-227.(Li M C,Cai Y,Zhao G L,et al.Improvement of cotyledon node regeneration system in soybean(Glycine max) [J].Acta Agronomica Sinica,2006,32(2):223-227.)
[3]王萍,王军军,商德虎,等.影响大豆子叶节丛生芽形成的诱导因子研究[J].吉林农业科学,2001,26(6):20-23.(Wang P,Wang J J,Shang D H,et al.Effect of induce factors on multiple bud formation of cotyledonary node in soybean[J].Journal of Jilin Agricultural Sciences,2001,26(6):20-23.)
[4]龚学臣,季静,王萍,等.苗龄与6-BA浓度对大豆子叶节丛生芽诱导的影响[J].吉林农业大学学报,2005,27(2):128-130.(Gong X C,Ji J,Wang P,et al.Effect of aseptic seeding age and 6-BA concentration on overgrowing shoots of soybean cotyledonary node[J].Journal of Jilin Agricultural University,2005,27(2):128-130.)
[5]刘北东,朱延明,李海燕,等.大豆子叶节再生影响因素的研究[J].大豆科学,2002,21(2):88-91.(Liu B D,Zhu Y M,Li H Y,et al.Study on factors affection the soybean cotyledonary node regeneration [J].Soybean Science,2002,21(2):88-91.)
[6]Liu H K,Yang C,Wei Z M.Efficient Agrobacterium tumefaciens-mediated transformation of soybeans using an embryonic tip regeneration system [J].Planta,2004,219:1042-1049.
[7]王萍,张淑珍,李文滨,等.大豆不同基因型胚尖不定芽的诱导及对抗生素的敏感性[J].作物杂志,2010(2):50-53.(Wang P,Zhang S Z,Li W B,et al.Induction of adventitious shoots from embryonic tip of different soybean gentotypes and their sensibility to antibiotics[J].Crops,2010 (2):50-53.)
[8]张艳,南相日,满为群,等.大豆离体培养及高频再生基因型的筛选[J].植物生理学通讯,2011,46(11):1135-1139.(Zhang Y,Nan X R,Man W Q,et al.Culture in vitro and high-frequency regeneration genotype selection of soybean[J].Plant Physiology Communications,2011,46(11):1135-1139.)
[9]邱承祥,武天龙.6-BA对大豆茎尖诱导再生植株的研究[J].大豆科学,2003,22(1):32-35.(Qiu C X,Wu T L.Study on 6-BA to the regeneration of tip shoot of soybean[J].Soybean Science,2003,22(1):32-35.)
[10]闫帆,孙昕,翟莹,等.6-BA浓度及基因型对大豆胚尖诱导丛生芽的影响[J].大豆科学,2011,30(1):29-32.(Yan F,Sun X,Zhai Y,et al.Effect of different 6-BA concentration and genotypes on shoots induced from embryonic tips[J].Soybean Science,2011,30(1):29-32.)[11]Kaneda Y,Tabei Y,Nishimura S,et al.Combination of thidiazuron and basal media with low salt concentrations increases the frequency of shoot organogenesis in soybeans [Glycine max ( L.) Merr.] [J].Plant Cell Report,1997,17:8-12.
[12]张晓娟,方小平,罗丽霞,等.TDZ和BA对诱导大豆胚轴植株再生的影响[J].中国油料作物学报,2000,22(1):24-26.(Zhang X J,Fang X P,Luo L X,et al.Influence of TDZ and BA on efficiency of plant regeneration via organogenesis in soybean[J].Chinese Journal of Oil Crop Sciences,2000,22(1):24-26.)
[13]李海燕,武小霞,刘淼,等.大豆子叶节、胚尖再生植株的研究[J].大豆科学,2007,26(5):709-712.(Li H Y,Wu X X,Liu M,et al.Plant regeneration from cotyledonary nodes and embryonic tips of soybean[J].Soybean Science,2007,26(5):709-712.)
[14]张艳,满为群,南相日,等.“黑农51”子叶节和胚尖再生体系的建立及优化[J].中国农学通报,2011,27(9):148-151.(Zhang Y,Man W Q,Nan X R,et al.Stablishment and improvment on regeneration systems of ‘Heinong51’ cotyledonary nodes and embryonic tips[J].Chinese Agricultural Science Bulletin,2011,27(9):148-151.)
[15]林树柱,曹越平,卫志明,等.6-BA诱导大豆子叶节和茎尖出芽的研究[J].上海交通大学学报(农业科学版),2005,23(2):138-142.(Lin S Z,Cao Y P,Wei Z M,et al.Studies on shoots induced by 6-BAfrom cotyledonary nodes and embryonic tips of soybean[J].Journal of Shanghai Jiaotong University(Agricultural Science),2005,23(2):138-142.)
[16]王伟,王罡,季静,等.大豆胚尖再生体系的优化及与子叶节再生体系的比较[J].大豆科学,2012,31(3):353-357.(Wang W,Wang G,Ji J,et al.Optimization of embryonic tip regeneration system and comparison with cotyledonary node regeneration system in soybean[J].Soybean Science,2012,31(3):353-357.)
[17]段莹莹,赵琳,陈李淼,等.农杆菌介导的大豆子叶节和下胚轴转化方法的比较及优化[J].大豆科学,2010,29(4):590-593.(Duan Y Y,Zhao L,Chen L M,et al.Comparison and optimization of the Agrobacterium-mediated transformation of soybean by using cotyledonary node and hypocotyl explants[J].Soybean Science,2010,29(4):590-593.)
[18]刘栋,石强,李鹏丽,等.利用GUS基因瞬时表达对大豆子叶节和胚尖转化方法的比较及优化[J].生物学通报,2008,43(12):35-39.(Liu D,Shi Q,Li P L,et al.Comparison and optimization of two kinds of transformation methods via cotyledonary node and embryonic tip using GUS gene transient expression in soybean[J].Bulletin of Biology,2008,43(12):35-39.)

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Last Update: 2014-12-25