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Effect of PEG Simulated Drought Stress on Nitrate Uptake and Root Tip Plasma Membrane H+-ATP Activity in Black Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年05期
Page:
674-679
Research Field:
Publishing date:

Info

Title:
Effect of PEG Simulated Drought Stress on Nitrate Uptake and Root Tip Plasma Membrane H+-ATP Activity in Black Soybean
Author(s):
PANG Xiao-luLIU AngCHEN Dong-jieCHEN Li-mei
Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650500,China
Keywords:
PEG simulated drought stressBlack beansDrought tolerancePlasma membrane H+-ATPase activityNitrate uptake
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0674
Abstract:
In this study,aluminum(Al)tolerant Tamba black soybean(RB)and Al-sensitive black soybean(SB)were used as experimental materials to analyze the physiological and biochemical characteristics of RB and SB in response to polyethylene glycol(PEG-6000)simulated drought stress under hydroponic conditions.The results showed that,under 2%,5%,10% PEG stress for 5 h and 2 d,the decreases in SB leaf transpiration rate and stomatal conductance were greater than those in RB.Stress in short period(5 h),RB and SB nitrate uptake were increased with the increasing PEG concentration,SB uptake of nitrate uptake was significantly higher than that under the same treatment condition RB.Under 5%PEG stress increased to 1,2,3 and 4 d,the nitrate uptake of RB and SB were still significant increase compared with control,but under the same stress conditions,RB nitrate absorption significantly exceeded the amount of absorption of the SB.The apical plasma membrane H+-ATPase activity and hydrogen pump activity of RB and SB with increasing PEG stress time showed a significant decreasing trend,but under the same conditions of stress treatments,the apical plasma membrane H+-ATPase activity and hydrogen pump activity of RB were significantly higher than SB.Those results suggested that SB drought-tolerance was stronger than RB,PEG simulated drought stress significantly enhanced the absorption of nitrate in RB and SB,but reduced the apical plasma membrane H+-ATPase activity and hydrogen pump activity.

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Last Update: 2014-12-25