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Expression Pattern of GmARF16 in Different Soybean Organs and Construction of Resistant Version Vector for GmARF16(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年05期
Page:
661-666
Research Field:
Publishing date:

Info

Title:
Expression Pattern of GmARF16 in Different Soybean Organs and Construction of Resistant Version Vector for GmARF16
Author(s):
LI Xiao-ping; ZEN Qing-fa; ZHAO Juan
College of Life Sciences,Huaibei Normal University/Anhui Plant Resource Key Lab,Huaibei,235000,China
Keywords:
Auxin response factors; Construction; MicroRNA; Expression analysis
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0661
Abstract:
By mining soybean and Arabidopsis genome data,we analyzed the interaction between auxin response factor GmARF16 and Gm-miR160,a kind of the microRNA and generated resistant version fragment mGmARF16.To looking for function of GmARF16,a binary vector pGmARF16∷mGmARF16 was constructed using mGmARF16 through Topo cloning and LR reaction.Moreover,real-time quantity PCR was used to investigate expression pattern of GmARF16 throughout the life span of soybean.GmARF16 was found to be -expressed at high levels in flowers and at low levels in stem.Interestingly,its transcriptional level was found higher in trifoliolate than either in cotyledons or in unifoliolate.

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Last Update: 2014-12-25