|Table of Contents|

Genome-wide Identification,EST Expression,and Upstream Sequence Analysis of CYS Family Genes in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年05期
Page:
654-660,666
Research Field:
Publishing date:

Info

Title:
Genome-wide Identification,EST Expression,and Upstream Sequence Analysis of CYS Family Genes in Soybean
Author(s):
XIE Ling12KIM Jeong-jun2WANG Zhang-xun3HUANG Bo3
1.School of Life Sciences,Anqing Teachers College,Anqing 246011,China;
2.Agricultural Microbiology Division,National Academy of Agricnltural Science,RDA,Sumon 441-707,Korea;
3.Anhui Provincial Key Laboratory for Microbial Pest Control,Anhui Agricultural University,Hefei 230036,China
Keywords:
Based on bioinformatic methodthe CYS family genes of soybean were identified and their sequencesstructures and chromosome location were determined.CYS proteins were classified according to their structure and phylogeny relationship.The results of genome analysis showed that a total of 19 CYS geneslocated on 12 chromosomesrespectivelycould be systematically identified from soybean genome and classified into three classes.All of them had stressed related cis-acting elements in their upstream sequences.The EST data of CYS family genes from the UniGene database was used to analyze the expression pattern of those genes in different tissues.EST analysis revealed that there was predominant expression of most soybean CYS family genes in cotyledonflowerleaf and seed coat.These results will facilitate functional analysis and utilization of CYS genes in soybean.Key words: SoybeanCYS family genesDifferential expressionPhylogenetic analysis
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0654
Abstract:
Based on bioinformatic method,the CYS family genes of soybean were identified and their sequences,structures and chromosome location were determined.CYS proteins were classified according to their structure and phylogeny relationship.The results of genome analysis showed that a total of 19 CYS genes,located on 12 chromosomes,respectively,could be systematically identified from soybean genome and classified into three classes.All of them had stressed related cis-acting elements in their upstream sequences.The EST data of CYS family genes from the UniGene database was used to analyze the expression pattern of those genes in different tissues.EST analysis revealed that there was predominant expression of most soybean CYS family genes in cotyledon,flower,leaf and seed coat.These results will facilitate functional analysis and utilization of CYS genes in soybean.

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Last Update: 2014-12-25