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Bioinformatics Analysis of WRKY31 Homologs in Soybean Genome(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年05期
Page:
642-647
Research Field:
Publishing date:

Info

Title:
Bioinformatics Analysis of WRKY31 Homologs in Soybean Genome
Author(s):
SUN Jing; SHENG Bi-han; HAN Ying-peng; ZHAO Xue; WANG Qiang; MENG Xian-xin; WEI Shu-hong; LI Wen-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education,Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry,Northeast Agricultural University,Harbin 150030,China
Keywords:
Soybean; WRKY31; Stress; WRKY domain; Phylogenetic
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0642
Abstract:
The gene family of WRKY is transcription factor that can be induced and involved in the response to biotic and abiotic stresses,and is conserved evolutionarily.In this research,the full-length cDNA of GmWRKY31 was cloned and bioinformatics analysis was used to predict the potential function of GmWRKY31.Phylogenetic analysis of GmWRKY31 and its homologs with different plant species were conducted by the software MEGA 5.0.The results showed that three copies of WRKY31 exited in the soybean genome,located on the chromosome 6,12 and 13.GmWRKY31 belongs to the type II WRKY transcription factors,with a WRKY domain and a C2H2-type zinc finger.Real-time RT-PCR analysis showed that GmWRKY31 was expressed in soybean leaf,flower,pod,root,nodule,and the flower had the highest expression,the lowest expression was in the pod.The result of the phylogenetic tree indicted that WRKY31 from Glycine max and Phaseolus vulgaris were the nearest in genetic relationship.While Glyma06g46420.2 and Glyma12g10350.1(the other two copies of WRKY31 of Glycine max) had closer relationship.The result of protein structure and function prediction showed that GmWRKY31 was a transcription factor being involved in the stress response of plant,however,the phylogenetic tree indicted that significant difference of the WRKY genes happened in evolution of soybean.

References:

［1］Guilfoyle T J.The structure of plant gene promoters[J].Genetic Engineering,1997,19:15-47.

[2]Ishiguro S,Nakamura K.Characterization of a cDNA encoding a novel DNA-binding protein,SPF1,that recognizes SP8 sequences in the 5’upstream regions of genes coding for sporamin and beta-amylase from sweet potato[J].Molecular and General Genetics,1994,244(6):563-571.
[3]Rushton P J,Somssich I E,Ringler P,et al.WRKY transcription factors[J].Trends in Plant Science,2010,15(5):247-258.
[4]Schmutz J,Cannon S B,Schlueter J,et al.Genome sequence of the palaeopolyploid soybean[J].Nature,2010,463(7278):178-183.
[5]Song Y,Ai C R,Jing S J,et al.Research progress on functional analysis of rice WRKY genes[J].Rice Science,2010,17(1):60-72.
[6]Zhang Y J,Wang L J.The WRKY transcription factor superfamily:its origin in eukaryotes and expansion in plants[J].BMC Evolutionary Biology,2005,5:1-12.
[7]Clive J.Global status of commercialized biotech/GM crops:2008［M］.New York:ISAAA,2008:4-5.
[8]Michael I P,Monetti C,Chiu A C,et al.Highly efficient site-specific transgenesis in cancer cell lines[J].Molecular Cancer,2012,11(1):89-99.
[9]Eulgem T,Rushton P J,Robatzek S,et al.The WRKY superfamily of plant transcription factors[J].Trends in Plant Science,2000,5(5):199-206.
[10]Maleck K,Levine A,Eulgem T,et al.The transcriptome of Arabidopsis thaliana during systemic acquired resistance[J].Nature Genetics,2000,26(4):403-410.
[11]Ulker B,Somssich I E. WRKY transcription factors:from DNA binding towards biological function[J].Current Opinion in Plant Biology,2004,7(5):491-498.
[12]王春梅.浅谈大豆高产病虫害的防治方法[J].科技致富向导,2014(8):11.(Wang C M.Discussion on prevention and treatment of diseases and insect pests of soybean yield[J].The Wizard Rich Technology,2014(8):11.)
[13]Xie Z,Zhang Z L,Zou X L,et al.Interactions of two abscisic-acid induced WRKY genes in repressing gibberellin signaling in aleurone cells[J].The Plant Journal,2006,46 (2):231-242.
[14]Zou X L,Neuman D,Shen Q J.Interactions of two transcriptional repressors and two transcriptional activators in modulating gibberellin signaling in aleurone cells[J].Plant Physiology,2008,148 (1):176-186.
[15]Ramamoorthy R,Jiang S Y,Kumar N,et al.A comprehensive transcriptional profiling of the WRKY gene family in rice under various abiotic and phytohormone treatments[J].Plant Cell Physiology,2008,49(6):865-879.
[16]Dong J,Chen C,Chen Z.Expression profiles of the Arabidopsis WRKY gene superfamily during plant defense response[J].Plant Molecular Biology,2003,51(1):21-37.
[17] Liu X,Bai X,Wang X,et al.OsWRKY71,a rice transcription factor,is involved in rice defense response[J].Journal of Plant Physiology,2007,164:969-979.
[18]仇玉萍,荆邵娟,付坚,等.13个水稻WRKY基因的克隆及其表达谱分析[J].科学通报,2004,49(18):1860-1869.(Qiu Y P,Jing S J,Fu J,et al.Clone and expression profiling analysis of 13 WRKY transcription factorsin rice[J].Chinese Science Bulletin,2004,49 (18):1860-1869.)
[19] Marè C,Mazzucotelli E,Crosatti C,et al.Hv-WRKY38:a new transcription factor involved in cold- and drought-response in barley[J].Plant Molecular Biology,2004,55:399-416.
[20]Seki M,Ishida J,Nanjo T,et al.Monitoring the expression profiles of 7000 Arabidopsis genes under drought,cold and high-salinity esses using a full-length cDNA microarray[J].The Plant Journal,2002,31(3):279-292.

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Last Update: 2014-12-24