|Table of Contents|

Study on Agrobacterium-mediated Transformation System of Super-high-yielding Soybean Cotyledon Node(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年05期
Page:
634-637
Research Field:
Publishing date:

Info

Title:
Study on Agrobacterium-mediated Transformation System of Super-high-yielding Soybean Cotyledon Node
Author(s):
JIA Yu-ying1JIANG Ying2ZHAO Qiang1XIE Fu-ti1YU Cui-mei1
1.College of Agronomy,Shenyang Agricultural University,Shenyang 110866,China;
2.College of Chemistry and Life Science,Shenyang Normal University,Shenyang 110034,China
Keywords:
Super-high-yielding soybeansAgrobacterium tumefaciensCotyledonary nodeTransformation
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.05.0634
Abstract:
To establish a high frequency genetic transformation system of soybean cotyledon node for soybean genetic engineering,Agrobacterium concentration,infection time,co-cultivation time and acetosyringone concentration of co-culture medium during soybean cotyledonary node transformation were studied by using soybean cultivars with super-high-yielding.The results showed that the optimal conditions were Agrobacterium concentration OD600=0.5,infection time 30 min,co-cultivation time 3-4 days and acetosyringone concentration 200 μmol.L-1.Using the optimized transformation procedure,different genotypes of super-high-yielding soybeans were transformed and the sensitivity of soybean genotypes to Agrobacterium tumefaciens was significant differently.Shennong 9 was the most sensitive material,followed by Shennong 12,and Zhonghuang 35 was the least sensitive material.

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Last Update: 2014-12-24