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Genetic Stability Analysis and Agronomic Traits Investigation of cry6Aa2m Transgenic Glycine max(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年05期
Page:
629-633
Research Field:
Publishing date:

Info

Title:
Genetic Stability Analysis and Agronomic Traits Investigation of cry6Aa2m Transgenic Glycine max
Author(s):
YANG Qiu-jiao1; 2; SUN Xiao-li2; SUN Ming-zhe2; CHEN Chao2; ZHAO Chao-yue2; JI Wei2; ZHU Yan-ming1; 2
1.School of Life Sciences,Tarim University,Alar 843300,China;
2.Key Laboratory of Agricultural Biological Functional Genes,Northeast Agricultural University,Harbin 150030,China
Keywords:
Transgenic soybean; Cry6Aa2m gene; Genetic stability; Agronomic traits
PACS:
S565.1
DOI:
10.11867/j.issn.1000-9841.2014.05.0629
Abstract:
In our previous studies,we had transformed the insect-resistant gene cry6Aa2m into Glycine max and obtained the T4 generation of the cry6Aa2m transgenic lines.In order to screen the transgenic soybean lines not only with stable expression of the cry6Aa2m gene but also sharing good agronomic traits,in the current study,we analyzed the genetic stability and investigated the agronomic traits of the transgenic soybean lines with gene cry6Aa2m.By using PCR and RT-PCR analysis,we verified the heredity and expression of the cry6Aa2m gene in the T4generation transgenic plants,which were investigated in this study.As expected,we showed over 85% PCR-positive plants of all the T4transgenic lines,and demonstrated the constitutive expression of the cry6Aa2m gene in the transgenic lines by using the semi-quantitative RT-PCR assays.In this aspect,we investigated and analyzed the agronomic traits of four transgenic lines(DpC6b4,DpC6b7,DpC6b8 and DpC6b37),including yield traits,morphological traits,quality traits and adaptive capacity to environment.Results of plant yield traits showed that all transgenic lines displayed no significant differences in the 100-seed weight and pods per plant,except for the significantly less pods per plant for line DpC6b7.As to the morphological trails,no significant differences were observed in growth period,pod bearing habit,flower color and leaf shape,except that the plant height of line DpC6b37 was significantly shorter than the non-transgenic soybean.Furthermore,we also found that the transgenic line DpC6b7 exhibited 4% higher in the protein content and 1% lower in the oil content than non-transgenic soybean,while line DpC6b37 displayed 2% and 5% higher,respectively.In addition,we also observed no obvious differences in germination rates,period of dormancy,weed species and quantity between transgenic and non-transgenic plants.Taken together,our results presented in this study suggested that the cry6Aa2m gene could be stably inherited and expressed in the transgenic Glycine max lines,and its overexpression did not affect plant agronomic traits.Among the four transgenic lines,DpC6b4 and DpC6b8,with relatively higher expression levels of the cry6Aa2m gene,displayed better agronomic traits than the non-transgenic plants,which made them as optimal candidates for further application in fields.

References:

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Last Update: 2014-12-24