Development of Visual Loop-Mediate Isothermal Amplification Assay for Herbicide-resistant Soybean GTS40-3-2 and Its Derivates(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2014年04期

Page:

570-573

Research Field:

Publishing date:

Info

Title:

Development of Visual Loop-Mediate Isothermal Amplification Assay for Herbicide-resistant Soybean GTS40-3-2 and Its Derivates

Author(s):

WANG Yong¹; 2; LAN Qing-kuo¹; ZHU Zhu¹; ZHAO Xin¹; 3; CHEN Rui¹; GUO Yong-ze¹; CHENG Yi¹

1.Tianjin Institute of Agricultural Quality Standard and Testing Technology,Tianjin 300381,China;

2.College of Plant Protection,Agricultural University of Hebei,Baoding 071001,China;

3.School of Chemical Engineering and Technology，Tianjin University，Tianjin 300072,China;

4.Tianjin Agricultural University,Tianjin 300384,China

Keywords:

Herbicide-resistant soybean GTS40-3-2; Event specific; Loop-mediated isothermal amplification; Visualization technology

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2014.04.0570

Abstract:

It is important to detect herbicide-resistant soybean GTS40-3-2 and its derivates in food or food materials in order to prevent the illegal diffusion.A rapid visual loop-mediated isothermal amplification(LAMP)method assay for GTS40-3-2 event-specific detection was developed.A set of primers were designed according to the nucleotide sequence of the target，8 genetically modified organisms(GMO)were detected by LAMP for method specificity，and meanwhile the mode of artificially contaminated food was constructed to evaluate the sensitivity of LAMP assay and qualitative PCR method.The results showed that the GTS40-3-2 event had specific amplification，but the non-CpTI GMO submitted negative reactions.Sensitivity of LAMP assay for GTS40-3-2 was 0.001%.In conclusion，the LAMP assay developed in the present study is a specific，sensitive，simple and convenient method for the rapid screening of GTS40-3-2 in contaminated foods.

References:

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