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Study on Physiological Characteristics of Selenite Uptake by Soybean Exised Roots(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年04期
Page:
519-523
Research Field:
Publishing date:

Info

Title:
Study on Physiological Characteristics of Selenite Uptake by Soybean Exised Roots
Author(s):
ZHANG Lian-he; YU Fei-yan; DENG Kun; XIE Yu-shong
Agricultural College,Henan University of Science and Technology,Luoyang 471003,China
Keywords:
Soybean excised roots; Selenite uptake; Physiological characteristics
PACS:
Q945.1
DOI:
10.11861/j.issn.1000-9841.2014.04.0519
Abstract:
Physiological characteristics of selenite uptake by soybean roots was investigated by means of physiological and pharmacological methods.The results showed that the rate of selenite uptake by soybean excised roots declined with an increase of pH.Concentration-dependent kinetics suggested that the rate of selenite uptake increased in proportion to the Se concentration in the absorption solution at pH3.0 and pH8.0.Linear equations were fitted to the data.The rate of selenite uptake increased and followed saturation kinetics with increasing Se concentrations in the absorption solution at pH5.0.The data fitted a Michaelis-Menten saturation curve.The results showed that DNP,NaF and a temperature of 4℃ repressed the rate of selenite uptake by 15%,19% and 23% at pH3.0,by up to 81%,80% and 79% at pH5.0,and by 16%,8% and 11% at pH8.0.Further study suggested that HgCl2 and AgNO3 inhibited selenite uptake by 78% and 79% at pH3.0.Phosphorus starvation increased the rate of selenite uptake by soybean roots at pH5.0.NPPB,9-CA,NPAA,TEACl,NA and DIDS inhibited selenite uptake by 13%,17%,22%,7%,18% and 6% at pH8.0.It demonstrated that selenite was absorbed passively by soybean roots through aquaporins in the form of H2SeO3 at pH3.0,and was absorbed actively through phosphorus transporters in the form of HSeO -3 at pH5.0.

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Last Update: 2014-09-11