|Table of Contents|

Exogenous SA Alleviated Al Toxicity by Inducing Citric Acid Exudation in Black Soybean Roots(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年04期
Page:
507-513
Research Field:
Publishing date:

Info

Title:
Exogenous SA Alleviated Al Toxicity by Inducing Citric Acid Exudation in Black Soybean Roots
Author(s):
WANG Wen-wen; CHEN Xuan-qin; CHEN Qi; CHEN Li-mei; LI Kun-zhi
Biotechnology Research Center,Kunming University of Science and Technology,Kunming 650500,Yunnan
Keywords:
Black beans; Al stress; Salicylic acid; Citric acid; 14-3-3; H+-ATPase
PACS:
S565.1；Q945
DOI:
10.11861/j.issn.1000-9841.2014.04.0507
Abstract:
A method of solution culture to Al-sensitive black soybean(SB)was used to study the effects of exogenous salicylic acid(SA)on the physiological parameters and Al stress-related gene expression in SB root under Al stress.The aim of the study is to explore the molecular mechanisms of exogenous SA alleviating Al toxicity in SB.The results showed that a low concentration(10 and 20 umol·L-1)of SA alleviated inhibition of root elongation caused by Al toxicity,wherein the alleviation effect of 20 umol·L-1 SA more significant.However,high concentration of SA and SA synthesis inhibitors paclobutrazol(PAC)increased inhibition of root elongation caused by Al toxicity.Exogenous 20 umol·L-1 decreased the MDA and H2O2 content in SB under Al stress,and the citric acid content in root exudates was about twice as higher as that of single Al treatment.Expression analysis showed that exogenous 20 umol·L-1 SA enhanced the expression of the 12 substype of 14-3-3 isoforms,plasma membrane H+-ATPase and multidrug and toxic compound extrusion transporter(MATE)gene in SB under Al stress.The results of the co-immunoprecipitation showed that exogenous 20 umol·L-1 SA improved the levels of protein phosphorylation for plasma membrane H+-ATPase and its binding capacity with 14-3-3 protein under Al stress.The effect of exogenous PAC treatment was opposite with SA.It was suggested that exogenous SA may enhance the SB tolerance to Al stress by inducing MATE expression and enhancing 14-3-3 and plasma membrane H+-ATPase protein levels and interaction between them to increase citric acid exudation.

References:

[1]Sasaki T,Yamamoto Y,Ezaki B,et al.A wheat gene encoding an aluminum-activated malate transporter[J].The Plant Journal,2004,37(5):645-653.

[2] 熊毅,李庆逵.中国土壤[M].北京:科学出版社,1987:39.(Xiong Y,Li Q K.Chinese soil[M].Beijing:Science Press,1987:39.)

[3] von Uexküll H R,Mutert E.Golbal extent development and economic impact of acid soils[J].Plant and Soil,1995,171(1):1-15.

[4] Matsumoto H.Cell biology of aluminum toxicity and tolerance in higher plants[J].International Review Cytology,2000,200:1-46.

[5] Scott R,Hoddinott J,Taylor G J,et al.The influence of aluminum on growth,carbohydrate and organic acid content of an Al-tolerant and Al-sensitive cultivar of wheat[J].Canadian Journal of Botany,1991,69(4):711-716.

[6] 郝林.水杨酸参与生物学过程的交谈机制[J].沈阳师范大学学报(自然科学版),2010,28(4):449-457.（Hao L.The conversation mechanisms of salicylic acid involved in biological processes[J].Journal of Shenyang Normal University(Natural Science),2010,28(4):449-457.）

[7] 李国靖,周燮.水杨酸与植物抗非生物胁迫[J].植物学通报,2001,18(3):295-302.（Li G J,Zhou X.Salicylic acid and abiotic stress resistance in plants[J].Chinese Bulletin of Botany,2001,18(3):295-302.）

[8] 彭陈,何冰,郭士伟.植物中的P-ATP酶[J].植物生理学通讯,2010,46(7):637-641.（Peng C,He B,Guo S W.P-ATPases in plants[J].Plant Physiology Communications,2010,46(7):637-641.）

[9] Yan F,Zhu Y,Müller C,et al.Adaptation of H+-Pumping and plasma membrane H+-ATPase activity in proteoid roots of white lupin under phosphate deficiency[J].Plant Physiology,2002,129(1):50-63.

[10]Shen H,He L F,Sasaki T,et al.Citrate secretion coupled with the modulation of soybean root tip under aluminum stress up-regulation of transcription,translation,and threonine-oriented phosphorylation of plasma membrane H+-ATPase[J].Plant Physiology,2005,138(1):287-296.

[11]Chung H J,Sehnke P C,Ferl R J.The 14-3-3 proteins:cellular regulators of plant metabolism[J].Trends in Plant Science,1999,4(9):367-371.

[12]Sehnke P C,Rosenquist M,Alsterfjord M,et al.Evolution and isoform specificity of plant 14-3-3 proteins[J].Plant Molecular Biology,2002,50(6):1011-1018.

[13]Tomasi N,Kretzschmar T,Espen L,et al.Plasma membrane H+-ATPase-dependent citrate exudation from cluster roots of phosphate-deficient white lupin[J].Plant,Cell & Environment,2009,32(5):465-475.

[14]Chen Q,Wu K H,Zhang Y N,et al.Physiological and molecular responses of broad bean(Vicia fabaL.)to aluminum stress[J].Acta Physiologiae Plantarum,2012,34(6):2251-2263.

[15]Aksamit A,Korobczak A,Skala J,et al.The 14-3-3 gene expression specificity in response to stress is promoter-dependent[J].Plant & Cell Physiology,2005,46(10):1635-1645.

[16]邵小杰,杨洪强,冉昆,等.水杨酸对镉胁迫下葡萄根系质膜ATPase和自由基的影响[J].中国农业科学,2010,43(7):1441-1447.（Shao X J,Yang H Q,Ran K,et al.Effects of salicylic acid on plasma membrane ATPase and free radical of grape root under cadmium stress[J].Scientia Agricultura Sinica,2010,43(7):1441-1447.）

[17]Liu N,You J,Shi W,et al.Salicylic acid involved in the process of aluminum induced citrate exudation in Glycine maxL.[J].Plant and Soil,2012,352(1-2):85-97.

[18]Neto A D,Prisco J T,Filho J E,et al.Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants[J].Journal of Plant Physiology,2005,162(10):1114-1122.

[19]张志良,瞿伟菁.植物生理学实验指导[M].3版.北京:高等教育出版社,2003:274-277.（Zhang Z L,Qu W J.The experimental guide for plant physiology[M].3rd ed.Beijing:Higher Education Press,2003:274-277.）

[20]李忠光,宋玉泉,龚明.二甲酚橙法用于测定植物组织中的过氧化氢[J].云南师范大学学报,2007,27(3):50-54.（Li Z G,Song Y Q,Gong M.Xylenol orange method used for the measurement of hydrogen peroxide in plant tissue[J].Journal of Yunnan Normal University,2010,43(7):1441-1447.）

[21]Foy C D,Carter T E,Duke J A,et al.Correlation of shoot and root growth and its role in selecting for alumium tolerance in soybean[J].Journal of Plant Nutrition,1993,16(2):305-325.

[22]Li X,Dhaubhadel S.Soybean 14-3-3 gene family:identification and molecular characterization[J].Planta,2011,233(3):569-582.

[23]Yang Z Y,Wang J,Wang S H,et al.Salicylic acid-induced aluminum tolerance by modulation of citrate efflux from roots of Cassia toraL.[J].Planta,2003,217(1):168-174.

[24]Wang Y S,Wang J,Yang Z M,et al.Salicylic acid modulates aluminum-induced oxidative stress in roots of Cassia tor[J].Acta Botanica Sinica,2004,46(7):819-828.

[25]高培培,章艺,吴玉环,等.外源水杨酸对Al胁迫下栝楼光合特性及耐Al性的影响[J].水土保持学报,2012,26(6):268-273.（Gao P P,Zhang Y,Wu Y H,et al.Effect of exogenous SA on photosynthesis and Al tolerance of Ttichosanthes kirilowiiMaxim under aluminum stress[J].Journal of Soil and Water Conservation,2012,26(6):268-273.）

[26]Zhao Z,Ma J F,Sato K,et al.Differential Al resistance and citrate secretion in barley(Hordeum vulgareL.)[J].Planta,2003,217(5):794-800.

[27]张士功,高吉寅,宋景芝.水杨酸和阿斯匹林对小麦盐害的缓解作用[J].植物生理学报,1999,25(2):159-164.（Zhang S G,Gao J Y,Song J Z.Mitigative effects of salicylic acid and aspirin on salt stress-induced injuries in wheat(Triticum aestivumL.)[J].Acta Phytophysiologica Sinica,1999,25(2):159-164.）

[28]郭春晓,郑成淑,谢红英,等.盐胁迫下外源水杨酸对菊花根系离子含量和ATPase和PPase活性的影响[J].园艺学报,2011,38(6):1167-1172.（Guo C X,Zheng C S,Xie H Y,et al.Effect of exogenous salicylic acid on ion content,activities of ATPase and PPase of the roots in chrysanthemum under salt stress[J].Acta Horticulturae Sinica,2011,38(6):1167-1172.）

[29]Chen F,Li Q,Sun L,et al.The rice 14-3-3 gene family and its involvement in responses to biotic and abiotic stress[J].DNA Research,2006,13(2):53-63.


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Last Update: 2014-09-11