Bioinformatics Analysis on 5′ Regulatory Region Sequence of DNA Demethylatase Gene IDM1 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2014年04期

Page:

483-487

Research Field:

Publishing date:

Info

Title:

Bioinformatics Analysis on 5′ Regulatory Region Sequence of DNA Demethylatase Gene IDM1 in Soybean

Author(s):

FANG Shu-mei¹; HAN Yi-qiang¹; ZHANG Wen-hui²; HONG Yan-hua²; LI Dan-dan²; FAN Jin-hui¹; LIANG Xi-long²

1.College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China；

2.Agronomy College,Heilongjiang Bayi Agricultural University,Daqing 163319,China

Keywords:

Soybean; DNA demethylatase; IDM1; Bioinformatics

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2014.04.0483

Abstract:

The condition of IDM1 gene in soybean was investigated,and the promoter sequences,methylation sites and transcription factor binding sites of 5′ regulatory region were analyzed by bioinformatics methods.The results indicated that IDM1 gene contained two copies of Glyma12g35760 and Glyma13g34641 in soybean and alternative splicing occurred during gene expression.Glyma12g35760 may had one promoter sequence,one CpG isoland with 133 bp length between 495-627 nucleotides.There were five potential transcription factor binding sites combined two transcription factors of SBF-1 and athb-1.Glyma12g35760 may contain eight promoter sequences but no CpG isoland.There were six potential transcription factor binding sites combined three transcription factors of P,SBF-1 and MYB.Ph.These results can provide very valuable information for experimental research of IDM1 gene in soybean.

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