The Target Genes Prediction and Analysis of gma-miR1508a (PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2014年04期

Page:

483-487

Research Field:

Publishing date:

Info

Title:

The Target Genes Prediction and Analysis of gma-miR1508a

Author(s):

LI Yong-guang¹; AI Jia¹; WANG Tao¹; JIN Long-guo²; QIU Li-juan²; LI Wen-bin¹

1.Chinese Education Ministry’s Key Laboratory of Soybean Biology & Agricultural Ministry’s Northeast Areal Key Laboratory of Soybean Biology and Genetic Breeding,Northeast Agricultural University,Harbin150030,China;

2.Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China

Keywords:

Soybean; miRNA; Promoter; Target gene

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2014.04.0479

Abstract:

microRNA(miRNA)is widely distributed RNA regulatory genes,endogenous and 21-25nt noncoding single-stranded small molecule RNA.miRNA has a complementary relationship with its target gene mRNA,and mediates the degradation of target genes.The transcriptional gene silencing causes the down-regulation of the expression of the target gene.In the present study,the promoter and the target gene of gma-miR1508a gene were predicted by bioinformatics methodology in PlantCARE,PMRD and BAR databases.Analysis results showed that many important abiotic stress cis-acting regulatory elements were found in the promoter sequence and gene expression may induce by ABA,low temperature and drought stresses.Seven gma-miR1508a target genes were identified by PMRD software,which involved in cell apoptosis,regulation of flowering,stress responses,cell wall modification.Finally,Glyma10g03840.1,Glyma16g27800.1,Glyma17g07280.1 totally five of them may be the target genes of gma-miR1508a under low temperature stress.

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