Analysis of Gene Expression Related to Antioxidant Activity in Black Soybean(Glycine max var)Sprouts(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2014年04期

Page:

479-482

Research Field:

Publishing date:

Info

Title:

Analysis of Gene Expression Related to Antioxidant Activity in Black Soybean(Glycine max var)Sprouts

Author(s):

LAI Ji-xiang¹; HE Cong-fen²; FANG Yun¹; ZHAO Ya³; WEI Shao-min¹; 3

1.School of Chemical and Material Engineering,Jiangnan University,Wuxi 214122,China;

2.School of Science,Beijing Technology and Business University,Beijing 100048,China;

3.Shanghai Jahwa United Co.Ltd,Shanghai 200082,China

Keywords:

Black soybean sprouts; Antioxidant activity; Gene expression; Microarray; Quantitative real-time PCR(qRT-PCR)

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2014.04.0473

Abstract:

The purpose of this study was to analyze the gene expression of black soybean(Glycine max var)sprouts with high antioxidant activity.Microarray and GO(Gene Ontology)term enrichment analysis were used to research the expressed genes,and the results were confirmed by qRT-PCR.Microarray analysis of expressed genes between sprouts(0.5 and 5.0 cm)showed that the most up-regulated genes in 0.5 cm sprouts belonged to GO:0006979(response to oxidative stress),in which ten genes were significantly highly expressed:BE823689.1_567,GMFL01-02-F14-R_381,GMFL01-03-G22-R_364,GMFL01-14-M12-R_553,GMFL01-51-M23-R_265,AW757007. 1_297,AW761420.1_260,BI788389.1_501, BQ273202.1_332 and GMFL01-10-I14-F_701.Those genes mainly control the synthesis of heat shock protein and antioxidant enzymes which related to intracellular oxidation metabolic balance.The results of qRT-PCR analysis regarding up-regulated genes were consistent with those of the microarray analysis,which indicated that the results were biologically reproducible and believable.

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