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Construction of Detection Method for Water soluble Isoflavone Content in Soybean Milk(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年03期
Page:
429-433
Research Field:
Publishing date:

Info

Title:
Construction of Detection Method for Water soluble Isoflavone Content in Soybean Milk
Author(s):
LI Yi-ran1; 2; ZHAO Li-qin2; LI Ai-ke1; LIU Shan1; YUN Ting-ting1; CHEN Hai-xu1; 2; QI Wen-tao1
1.Academy of State Administration of Grain,Beijing 100037,China;2.College of Food Science and Engineering,Inner Mongolia Agricultural University,Hohhot 010018,China
Keywords:
HPLC; Isoflavone; Soybeanmilk
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.03.0429
Abstract:
The method for the detection of isoflavone in soybean milks by the High Performance Liquid Chromatography（HPLC）was optimized.And ultra high-speed centrifugation was used for the determination of watersoluble isoflavone contents including Daidzin,Genistin,Daidein and Genistein.The result showed that under the conditions of a C18 column(150 mm × 4.6 mm,5.0 μm),mobile phase A containing 0.1%(V/V)acetic acid in ultrapure aqueous,mobile phase B containing 0.1%(V/V)acetic acid in methanol,a gradient mobile phase at 260 nm,a flow rate of 0.8 mL?min-1,the four different kinds of isoflavones in soybeanmilk could be well separated and detected which good linearities with the correlation coefficient of daidzin,genistin,daidein,genistein were 0.999 2，0.999 1，0.999 3 and 0.9994,respectively.The result also showed that there were significant differences among the four kinds of isoflavone contents in different types of soymilk product.Furthermore,homemade soymilk has a significantly higher isoflavone content than common commercially soymilk.The method constructed can be used for the fast and accurate detection of isoflavone in different kinds of soybean milk.

References:


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Last Update: 2014-08-01