|Table of Contents|

Rapid Detection of Soybean Mosaic Virus by Reverse Transcription Loop Mediated Isothermal Amplification(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年03期
Page:
422-428
Research Field:
Publishing date:

Info

Title:
Rapid Detection of Soybean Mosaic Virus by Reverse Transcription Loop Mediated Isothermal Amplification
Author(s):
ZHANG Wen-na1; LI Jin-yu1; TIAN Jin-yan1; WEI Qi-wei1; ZHI Hai-jian2; TAO Xiao-rong1
1.Plant Protection College of Nanjing Agricultural University,Nanjing 210095,China;2.Soybean Research Institute,Nanjing Agricultural University/ National Key Laboratory for Crop Genetics and Germplasm Enhancement,National Center for Soybean Improvement,Nanjing 210095,China
Keywords:
Rapid detection; Soybean mosaic virus; RTLAMP; Soybean; Detection of infected seeds
PACS:
S435.651
DOI:
10.11861/j.issn.10009841.2014.03.0422
Abstract:
Soybean mosaic virus(SMV)is a widely distributed and highly destructive plant virus worldwide.Infection by SMV usually results in severe yield losses and seed quality reduction.SMV infected disease has caused a huge economic losses in many countries.There fore,reversetranscription loopmediated isothermal amplification(RTLAMP),a method to achieve rapid,sensitive and accurate detection of SMV in soybean leaves and seeds was established.Using SMV coat protein gene sequences from GenBank database,the RTLAMP primers sets were designed and the best primers set was selected.Then they were used for the optimization of the RTLAMP parameters.The sensitivity of the RTLAMP was 10100 times higher than the conventional RTPCR.When crude RNA was integrated in this assay,the developed onestep RTLAMP could rapidly and sensitively detect SMV in soybean leaves.The reaction product could be directly visualized in tube by adding the fluorescent dye SYBR I.The developed RTLAMP could also successfully detect SMV in soybean seeds.This is the first report of the application of the RTLAMP assay to detect SMV in soybean leaves and seeds.The rapid,sensitive and specific RTLAMP assay developed in this study can be applied widely in laboratories and the field detection of SMV.

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Last Update: 2014-08-01