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Isolation and Analysis on Ability of Phosphate Dissolving Bacteria in Rhizosphere of Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年03期
Page:
404-407
Research Field:
Publishing date:

Info

Title:
Isolation and Analysis on Ability of Phosphate Dissolving Bacteria in Rhizosphere of Soybean
Author(s):
WANG Hao; JIANG Yan; LIU Wei; LI Yuan-ming; YU Jia; WANG Shao-dong
National Research Center of Soybeen Engineering and Technology,Northeast Agricultural University,Harbin 150086,China
Keywords:
Soybean; Rhizosphere; Phosphate dissolving bacteria; Phosphate dissolving ability
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.03.0404
Abstract:
By using inorganic phosphate synthetic culture media,223 strains of inoganic phosphate dissolving bacteria were isolated from soybean rhizoshpere,47 strains could produce dissolving phosphate zone among them,and adding 1% Ca3(PO4)2 to soil was benefit to isolate strains with high ability of dissolving phosphate.The dissolving inorganic phosphate quantities measured were ranged from 51.64~199.20 mg.L-1.Passage cultured the most powerful dissolving phosphate strain SR95 and improved the content of Ca3(PO4)2in the media continuously,the dissolving inorganic phosphate quantities were improved from 199.20 to 243.71 mg.L-1.Sequenced partial 16S rDNA of the more powerful dissolving phosphate strains,nucleic acid sequence was blasted in GenBank online and phylogenetic tree was constructed.The result showed that all strains were in 7 different species,the most powerful dissolving phosphate strain SR 95 was identified as Pantoea agglomerans,which was not reported as dissolving phosphate bacteria previously.

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Last Update: 2014-08-01