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¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2014Äê03ÆÚ

Page:

379-384

Research Field:

Publishing date:

Info

Title:

Isolation and Identification of Rhizobium HD001 and Its Nodulation Capacity Test in Soybean Germplasm

Author(s):

WANG Hong-guang¹; SUN Dian-jun²; MA Zhong-qiang³; XIN Da-wei¹; WANG Jin-hui¹; LIU Chun-yan²; HU Guo-hua²; CHEN Qing-shan¹

1.Chinese Education Ministry¬ðs Northeast Key Laboratory of Soybean Biology,Agricultural Ministry¬ðs Area Key Laboratory of Soybean Biology and Genetic Breeding,Northeast Agricultural University,Harbin 150030,China;2.Land Reclamation Research and Breeding Centre of Heilongjiang,Harbin 150050,China;3.Beidahuang Kenfeng Seed Corporation,Harbin 150088,China

Keywords:

Soybean; Rhizboium; Isolation; Identification; Nodulation; Soybean germplasm

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2014.03.0379

Abstract:

In this study,the nodule of Suinong 14 were used to isolate new rhizobium strain.A cold field type rhizobium was isolated and identified.This rhizobium had high nodulation capacity in Suinong 14,which named as HD001.The secretome of type 3 effectors,support that HD001 is one special rhizobium of cold field area.Compared to HH103,there were 4 protein bands had difference at least.By the nodulation test on 218 soybean germplasm,the high nodule number and low nodule number phenotype varieties were identified.Ten high nodulation capacity germpalsm and eleven low nodulation capacity germplasm were found.These germplasm had a good meaning for the further sduty of mechanism to the symbiosis of soybean and rhizobium.

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