|Table of Contents|

Drought Resistance Evaluation of ?AtCBF4 ?Transgenic Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年03期
Page:
365-369
Research Field:
Publishing date:

Info

Title:
Drought Resistance Evaluation of ?AtCBF4 ?Transgenic Soybean
Author(s):
WANG Xing-yu12WEI Lai2WANG Wei-wei2XUE Yong-guo2TANG Xiao-fei2WU Guang-xi2WANG Peng-fei2YU Zhi-yuan1LIU Li-jun2
1.College of Life Science,Northeast Agricultural University,Harbin 150030,China;2.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China
Keywords:
SoybeanAtCBF4Transgenic strainsDrought resistance
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.03.0365
Abstract:
In this paper,we explored the performance of transgenic soybeans in photosynthetic characteristics of soybean(net photosynthetic rate,stomatal conductance,CO2 concentration intercellular,transpiration rate,instantaneous water use efficiency),physical characteristics(Pro,MDA,soluble sugar)and yield traits(plant height,number of sections,the number of pods per plant,seed number per plant,seed weight)under drought stress.Comprehensive evaluate the difference in droughttolerance among 7 soybean lines.Compared of transgenic lines and normal lines,the research showed that the contents of soluble sugar(WSS)and free proline(Pro)from transgenic lines were higher than normal ones,while less increment of Malondialdehyde(MDA)appeared.The transgenic lines had little change in photosynthetic characteristics and yield traits from before to under drought resistance.All above showed that compared with the normal,all transgenic soybeans have more adjustment for water deficit in pod stage and podfilling stage.HTCB59-5,HTCB59-2 and HTCB59-4 have more drought tolerance than others.

References:

[1] 李景新. 我国东北半干旱地区水土资源李景新的现状与发展对[J]. 农机使用与维修,2012 (6 ):103-104.(Li J X.Semi arid region of northeast China’s land and water resources situation and development strategy[J].Farm Machinery Using & Maintenance,2012(6):103-104.)
[2] 杜丽璞,徐惠君,叶兴国,等. 小麦转?TPS?基因植株的获得及其初步功能鉴定[J]. 麦类作物学报,2007 ,27(3) :369-373.(Du L P,Xu H J,Ye X G,et al.Transgenic wheat plants with Trehalose 6 phosphate synthase(TPS)gene and identification of their function[J].Journal of Triticeae Crops,2007,27(3):369-373.) [3] 王晓飞, 程宪国, 王迎波, 等.Floraldip 法大豆?GmDREB?转录因子转拟南芥研究[J]. 生物技术通报,2008(5):103-107.(Wang X F,Cheng X G,Wang Y B,et al.Study on transgenic Arabidopsis with a soybean transcription factor gene GmDREB by Floraldip method[J].Biotechnology Bulletin,2008(5):103-107.)
[4] 高世庆,徐慧君. 转大豆?GmDREB?基因增强小麦的耐旱及耐盐[J]. 科学通报,2005 ,50(23) :2617-2625.(Gao S Q,Xu H J.Soybean GmDREB gene transfer enhanced drought and salt tolerance of wheat[J].Chinese Science Bulletin,2005,50(23):2617-2625.)
[5]Volker H ,Daniel C ,Jose L R ,et al.Transcription factor CBF4 is are gulator of drought adaptation in Arabidopsis[J].Plant Physiology,2002,130:639-648.
[6] 林茂,闫海霞,眭顺照等. 植物CBF 转录因子及其在基因工程中的应用[J]. 广西农业科学,2008,39(1):21-25.(Lin M,Yan H X,Sui S Z,et l.CBF transcription factors of plant and its application in genetic engineering[J].Guangxi Agricultural Sciences,2008,39(1):21-25.)
[7]Jaglo Ottosen K R ,Gilmour S J ,Zarka D J G ,et al.Arabidopsis CBF1 over expression induces COR genes and enhances freezing tolerance[J].Science,1998,280:104-106.)
[8] 张晗,信月芝,郭惠明等.CBF 转录因子及其在植物抗冷反应中的作用.[J]. 核农学报,2006 ,20(5 ):406-409.(Zhang H,Xin Y Z,Guo H M,et al.CBF transcriptional factors and its roles in plant freezing tolerance[J].Journal of Nuclear Agricultural Sciences,2006,20(5):406-409.)
 [9] 吴广锡, 刘丽君, 杨德光, 等. 转GmHsFA1基因大豆的耐热性评价[J]. 大豆科学,2012 ,31(3):341-346.(Wu G X,Liu L J,Yang D G,et al.Heat resistance evaluation of GmHsFA1 [J].Soybean Science ,2012,31(3):341-346.)
[10] 王树宇, 付连舜, 刘威. 野生大豆资源抗旱性鉴定与应用分析[J]. 中国农业,2013 (6 ):55-57.(Wang S Y,Fu L S,Liu W,et al.Drought resistance identification and application analysis in wild soybean[J].Chinese Agriculture,2013(6):55-57.)
[11] 张海燕, 焦碧婵, 李贵全. 大豆抗旱性鉴定指标评价的研究[J]. 大豆科学,2005 ,24 (3 ):183-188. (Zhang H Y,Jiao B C,Li G Q.Study on selecting targets in drought resistant breeding of soybean[J].Soybean Science,2005,24(3):183-188. )
[12] 孔照胜,武云帅,岳爱琴,等. 不同大豆品种抗旱性生理指标综合分析[J]. 华北农学报,2000 ,16(3):40-45.(Kong Z S,Wu Y S,Yue A Q,et al.Comprehensive analysis of physiological indexes for drought resistance in different soybean varieties[J].Acta Agriculturae Boreali Sinica,2000,16(3):40-45.)

Memo

Memo:
-
Last Update: 2014-08-01