|Table of Contents|

Bioinformatics Analysis of DNA Demethylase ROS1 in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年03期
Page:
321-324
Research Field:
Publishing date:

Info

Title:
Bioinformatics Analysis of DNA Demethylase ROS1 in Soybean
Author(s):
LIANG Xi-long1; LI Guo-lan1; CUI Hong-qiu1; JU Shi-jie1; HONG Yan-hua1; FANG Shu-mei2; ZHENG Dian-feng1
1.Agronomy College,Heilongjiang Bayi Agricultural University,Daqing 163319,China;2.College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing 163319,China
Keywords:
Soybean; DNA demethylase; ROS1; Bioinformatics analysis
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.03.0321
Abstract:
Websites and data bases were employed such as NCBI,Phytozome and ExPASy in this paper to identify the sequences of proteins and genes,gene copies and some physicochemical properties of ROS1 in soybean.Its secondary structure and domain were predicted and analyzed.Meanwhile,phylogenetic tree was constructed based on the sequences of amino acids to analyze the revolutionary relations among different species.The results showed that ROS1 included six copies with similar molecular weight in soybean.There were acid isoelectric points and similar potential phosphorylation sites in the ROS1 proteins but signal sequences were lacked.All copies were all unstable and hydrophobic and were expressed in nucleus.α helix was the main secondary structure.DNA_glycosylase，HhH-GPD_domain and HTH_base_excis_C domains were included in the ROS1 proteins of soybean.

References:


[1]Zhu J K.Active DNA demethylation mediated by DNA glycosylases[J].Annual Review of Genetics,2009,43:143-166.
[2]Finnegan E J,Genger R K,Peacock W J,et al.DNA methylation in plants[J].Annual Review of Plant Biology,1998,49(1):223-247.
[3]Wada Y,Miyamoto K,Kusano T,et al.Association between up regulation of stress responsive genes and hypomethylation of genomic DNA in tobacco plants[J].Molecular Genetics and Genomics,2004，271,658-666.
[4]pan Y J,Fu B Y,Wang D,et al.Spatial and temporal profiling of DNA methylation induced by drought stress in rice[J].Scientia Agricultura Sinica,2009,42,3009-3018.
[5]Finnegan E J,Peacock W J,Dennis E S.DNA methylation,a key regulator of plant development and other processes[J].Current Opinion in Genetics & Development,2000,10(2):217-223.
[6]Jost J P,Saluz H P.DNA methylation:Molecular biology and biological significance[M].Basel:Birkhauser Verlag,1993:218-261.
[7]方淑梅，梁喜龙，纪伟波，等.大豆DNA甲基化酶生物信息学分析[J].中国油料作物学报，2013，35（2）：225-230.(Fang S M,Liang X L,Ji W B,et al.Bioinformatics analysis of DNA methylase from soybean[J].Chinese Journal of Oil Crop Sciences,2013,35(2):225-230.)
[8]范晓军，宋志芳，仙笑笑，等.金纹细蛾几丁质酶基因生物信息学分析[J].基因组学与应用生物学，2013，32（1）：91-96.(Fan X J,Song Z F,Xian X X,et al.Bioinformatical analysis of chitinase gene from Lithocolletics ringoniella[J].Genomics and Applied Biology,2013,32(1):91-96.)
[9]Agius F,Kapoor A,Zhu J K.Role of the Arabidopsis DNA glycosylase/lyase ROS1 in active DNA demethylation[J].Proceedings of the National Academy of Sciences,2006,103(31):11796-11801. [10]Gehring M,Reik W,Henikoff S.NA demethylation by DNA repair[J].Trends in Genetics,2009,25(2):82-90.
[11]Gong Z,MoralesRuiz,Ariza R R,et al.ROS1,a repressor of transcriptional gene silencing in Arabidopsis,encodes a DNA Glycosylase/Lyase[J].Cell,2002,111(6):803-814.

Memo

Memo:
-
Last Update: 2014-08-01