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Cloning and Functional Analysis of a Promoter of GmPLP1 Gene in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年03期
Page:
309-314
Research Field:
Publishing date:

Info

Title:
Cloning and Functional Analysis of a Promoter of GmPLP1 Gene in Soybean
Author(s):
LI Yong-guang; ZHANG Yu-hang; LUO Qiu-lan; GU Han-qing; LI Wen-bin
Key Laboratory of Soybean Biology in Chinese Ministry of Education,Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry,Northeast Agricultural University,Harbin 150030,China
Keywords:
GmPLP1 promoter; GUSgene; Histochemical staining; Cisregulatory elements
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.03.0311
Abstract:
To reveal the molecular mechanism underlying the expression of soybean PLP1(GmPLP1)gene that contains PAS/LOV domain,its 5′ regulatory region was analyzed.The1.5 kb fragment upstream of the GmPLP1 gene was isolated from soybean cultivar ‘Dongnong L13’by genome walking.There were 107 cisacting elements in the promoter predicted by online software Plant CARE and PLACE.the promoter fragment deletions were inserted into the 5′ region of the gus.A reporter gene in vector pBI121(replacing the 35S promoter).In order to determine the promoter characteristic of GmPLP1.The constructs were transferred into Nicotiana tabacum and Glycine max.The GUS activity was upregulated under dark,blue light,gibberellin A3 and abscisic acid driven by GmPLP1promoter.

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Last Update: 2014-08-01