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Mangrove endophytic Bacillus amyloliquefaciens AiL3 were used as materials to study the colonization and promotion growth in soybean.Mutant strains AiL3Rif owned stable marker of antibiotic rifampicin and inhibitory effect against Phytophthora sojae were screened by gradually increasing the concentration of antibiotics and dual culture method.Colonizing dynamics of the AiL3Rif strain in root,stem and leaf of soybean were investigated by treating with root pouring and leaf coating and promotion growth of plant were determined after root pouring inoculation.The results indicated that the strain AiL3Rif was remain stable resistance marker after 10 generations of cultivation.The inhibition rates of the original AiL3 and mutated AiL3Rif strains against P.sojae were 57.58% and 56.92%,respectively.The strain AiL3Rif could not only colonize in soybean but also conduct from top to bottom and effective colonization period could sustain 30 days or more.The most amount of the marked strain were 6.2×103 cfug-1?after 11 days inoculation of pouring inoculation and the colonizing ability in the root was stronger than stems and leaves.Treated with leaf coating,the most amount appeared in the leaves was 3.34×103 cfug-1,and followed by stems and least in the roots.The amount of bacteria colone of root pouring was higher than that of leaf coating and relatively stable.In soybean growthpromoting aspects,the content of 1.0×107cfumL-1?AiL3 fermentation broth were significantly higher than the other test groups based on determination results of the fresh and dry weight,height,root length,chlorophyll and root activity.It illustrated that mangrove endophytic bacteria AiL3 owned a obvious promoting effect for soybean.
[1]Tyler B M.Phytophthora sojae:root rot pathogen of soybean and model oomycete[J].Molecular Plant Pathology,2007,8:1-8.
[2]Wrather J A ，Stienstra W C ，Koenning S R.Soybean disease loss estimates for the United States from 1996 to 1998[J].Canadian Journal of Plant Pathology,2001,3:122-131.
[3]Hildebrand A A.A root and stalk rot caused by Phytophthorama var.sojae[J].Canadian Journal of Botany,1959,37:927-957.
[4]Pegg K G,Kochman J K,Vock N T.Root and stem rot of soybean caused by Phytophthora megasperma var.sojae in Australia[J].Australasian Plant Pathology,1980,9:15.
[5] 沈崇尧 , 苏彦纯 . 中国大豆疫霉菌的发现及初步研究[J]. 植物病理学报 ,1991,21(4):298.(Shen C Y ， Su Y C.Discovery and preliminary studies of Phytophthora megasperma-on soybean in China[J].Acta Phytopathologica Sinica,1991,21(4):298.)
[6] 杨光红 . 大豆疫霉拮抗菌株的筛选、鉴定及拮抗作用[D]. 合肥：安徽农业大学 ,2012.(Yang G H.Screening and identification of microorganisms against Phytophthora sojae and their antagonistic activity[D].Hefei:Anhui Agricultural University,2012.)
[7] 靳立梅，徐鹏飞，吴俊江，等 . 野生大豆种质资源对大豆疫霉根腐病抗性评价[J]. 大豆科学 ,2007 ， 26(3):300-304.(Jin L M,Xu P F,Wu J J,et a1.Identification the resistance of wild soybean germplasm to Phytophthora sojae[J].Soybean Science,2007,26(3):300-304.)
[8] 申宏波 , 姚文秋 , 于永梅 , 等 . 不同类型生物农药对大豆疫霉根腐病的防治效果[J]. 大豆科学 ,2011,30(6):1054-1056.(Shen H B,Yao W Q,Yu Y M,et al.Control efficiency of different biopesticides on soybean Phytophthora root rot[J].Soybean Science,2011,30(6):1054-1056.)
[9] 韩祥东 , 花美娜 , 冯永君 , 等 . 拮抗大豆疫霉菌植物内生细菌的筛选与鉴定[J]. 大豆科学 ,2012,31(1):85-91.(Han X D,Hua M N,Feng Y J,et al.Isolation and characterization of endophytic bacteria for Phytophthora sojae inhibition from soybean plant[J].Soybean Science,2012,31(1):85-91.)
[10] 付红梅 , 李淼 , 檀根甲 , 等 . 大豆疫霉拮抗菌株的筛选与鉴定[J]. 安徽农业科学 ,2011,39(19):11482-11484,11495.(Fu H M,Li M,Tan G J,et al.Screening and identification of antagonistic strain against Phytophthora sojae[J].Journal of Anhui Agricultural Sciences,2011,39(19):11482-11484,11495.)
[11] 陈宝如 , 詹儒林 , 何红 , 等 . 红树内生细菌 AiL3 菌株鉴定及其胞外抗菌活性物质特性 [J]. 农业生物技术学报 ,2010,18(4):801-806.(Chen B R,Zhan R L,He H,et al.Identification of endophytic bacteria AiL3 from mangrove and characterization of its active metabolites[J].Journal of Agricultural Biotechnology,2010,18(4):801-806.)
[12] 柳凤 , 何红 , 詹儒林 , 等 . 拮抗芒果炭疽病菌的红树内生细菌筛选及 AiL3 菌株抗菌物质研究[J]. 中国生物防治学报 ,2010,26(3):293-299.(Liu F,He H,Zhan R L,et al.Screening of mangrove endophytic bacteria against Colletotrichum gloeosporioides and characterization of antifungal substance produced by strain AiL3[J].Chinese Journal of Biological Control,2010,26(3):293-299.)
[13] 李合生 . 植物生理生化实验原理和技术 [M]. 北京 : 高等教育出版社 ,2000:119-120,134-137.(Li H S.Principles and techniques of plant physiological biochemical experiment[M].Beijing:Higher Education Press,2000:119-120,134-137.)
[14] 胡伟 , 赵兰凤 , 张亮 , 等 . 香蕉枯萎病生防菌 AF11 的鉴定及其定殖研究[J]. 中国生物防治学报 ,2012,28(3):387-393.(Hu W,Zhao L F,Zhang L,et al.Identification and colonization of bacteria AF11 antagonistic against Fusarium oxysporum f.sp.cubense race 4,the pathogen of banana wilt disease[J].Chinese Journal of Biological Control,2012,28(3):387-393.)
[15] 辛海峰 , 孟艳艳 , 李建宏 , 等 . 一株萎缩芽孢杆菌在小麦中的定植及对赤霉病的防治[J]. 生态学杂志 ,2013,32(6):1490-1496.(Xin H F,Meng Y Y,Li J H,et al.Bacillus atrophaeus strains colonization in wheat plant and its inhibition efficiency to Fusarium head blight[J].Chinese Journal of Ecology,2013,32(6):1490-1496.)
[16] 文才艺 , 赵凯旋 , 汪敏 , 等 . 内生细菌 EBS05 在小麦体内的定殖动态及其对小麦纹枯病的防治作用 [J]. 植物保护学报 ,2011,38(6):481-486.(Wen C Y,Zhao K X,Wang M,et al.Colonization trends of endophytic bacteria EBS05 in wheat and its control on wheat shape eyespot[J].Acta Phytophylacica Sinica,2011,38(6):481-486.)
[18] 蔡学清 , 林彩萍 , 何红 , 等 . 内生枯草芽孢杆菌 BS-Z 对水稻苗生长的效应[J]. 福建农林大学学报 ( 自然科学版 ),2005,34(2):189-194.(Cai X Q ， Lin C P,He H,et al.Effects of endophytic bacterial strain BS-2 on rice seeding growth[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition,2005,34(2):189-194.)
[19] 辜运富 , 张云飞 , 张小平 . 一株抗玉米纹枯病内生细菌的分离鉴定及其抗病促生作用[J]. 微生物学通报 ,2008,35(8):1240-1245.(Gu Y F,Zhang Y F,Zhang X P.Isolation and identification of one anti-Rhizoctonis solani endophytic bacteria strain from corn and its antagonism and promoting research[J].Microbiology,2008,35(8):1240-1245.)
[20]Deepa C K,Dastager S G,Pandey A.Plant growth-promoting activity in newly isolated Bacillus thioparus(NIi-0902)from western ghat forest,India[J].World Journal of Microbiology and Biotechnology,2010,26:2277-2283.