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Identification Efficiency about Resistance to Soybean Cyst Nematode with Relative Molecular Markers in Hybrid Progeny(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年02期
Page:
173-178
Research Field:
Publishing date:

Info

Title:
Identification Efficiency about Resistance to Soybean Cyst Nematode with Relative Molecular Markers in Hybrid Progeny
Author(s):
MA Yansong12LIU Xinlei1LUAN Xiaoyan1WANG Jiajun1LI Jinrong1YU Baishuang1QIU Lijuan2
1.Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;2.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China
Keywords:
Soybean cyst nematodeResistant identificationGenotypeDetection efficiency
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.02.0173
Abstract:
The restriction of identification condition and method is one of the important limiting factors for SCN germplasm screening and breeding.Using molecular marker closely linked with resistant gene is an efficient and accurate method to identify the resistance to SCN.In this study,we adopted several generation of segregation populations derived from hybridization between SCN resistant and susceptible parents to evaluate the identification efficiency of molecular markers.The ratio of individuals resistant to SCN in segregation population enhanced with the generation increasing.Chi-test showed the resistance to SCN was controlled by two recessive genes in segregation population.The detection efficiency of two Indel markers based on Rhg1 sequence,rhg1-I4 and SCN_Res Bridge,was 88.89%,and higher than that of Satt309(50.00%).With the increasing of breeding generation,the molecular markers detecting efficiency improved.The combination between different markers didn’t add more accuracy.We could anticipate that the markers designed by resistant sequence were new pathway to accelerate the SCN resistance breeding.

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Last Update: 2014-08-04