|Table of Contents|

Improvement of Soybean Hairyroot System Mediated by Agrobacterium Rhizogenes(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年02期
Page:
161-167
Research Field:
Publishing date:

Info

Title:
Improvement of Soybean Hairyroot System Mediated by Agrobacterium Rhizogenes
Author(s):
XU Jian-mei; YANG Qiao; LI Shuo; LIU Qian; JIAN Heng
Department of Plant Pathology,China Agricultural University,Beijing 100193,China
Keywords:
Soybean; Agrobacterium rhizogenes K599; RNA interference(RNAi); Genetic transformation
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.02.0161
Abstract:
In consideration of the fact that it is difficult to acquire transgenic soybean plants,we established a transformation system of soybean mediated by Agrobacterium rhizogenes K599 according to the method of Kereszt et al.,and improved it because of some specific questions in experiment.The improved transformation system and the original one were compared.Take advantage of the improved system and RNAi technology,transgenic hairy root plants that expressing nematode dsRNA of specific genes were constructed and the RNAi effects of these genes could be tested within 60 days on the transgenic hairy roots.The results indicate that the improved system is easier to manipulate,have less demands to environment conditions and personnel skilling levels,and improve the vitality of acquired plants.Our results established the foundation for breeding new crop cultivars with highly resistant to soybean cyst nematode.

References:

[1]Lodhi A H,Bongaerts R J M,Verpoorte R,et.al.Expression of bacterial isochorismate synthase in transgenic root cultures of Rubia peregrine[J].Plant Cell Reports,1996,15:54-57.
[2]Fujimoto Y,Ohyama K,Nomura K,et.al.Biosynthesis of sterols and ecdysteroids in Ajuga hairy roots[J].Lipids,2000,35:279- 288.
[3]Sevon N,Oksman C,Kirsi M.Agrobacterium rhizogenes mediated transformation:root cultures as a source of alkaloids[J].Planta Medica,2002,68:859-868.
[4]Moyano E,Jouhikainen K,Tammela P.Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoxcyamus muticus[J].Journal of Experimental Botany,2003,54:203-211.
?[5]Xiao K,Zhang C,Harrison M,et.al.Isolation and characterization of a novel plant promoter that directs strong constitutive expression of transgenes in plants[J].Molecular Breeding,2005,15:221-231.
[6]Ivashuta S,Liu J,Liu J,et al.RNA interference identifies a calcium-dependent protein kinase involved in Medicago truncatula root development[J].Plant Cell,2005,17:2911-2921.
[7]Cho H J,Wildholm J M.Improved shoot regeneration protocol for hairy roots of legume Astragalus sincus[J].Plant Cell Tissue Organ Culture,2002,69:259-269.
[8]Chaudhuri K N,Ghosh B,Tepfer D,et.al.Spontaneous plant regeneration in transformed roots and calli from Tylophora indica:changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes[J].Plant Cell Reports,2006,25:1059-1066.
?[9]Hansen A C,Busk H,Marcker A K,et.al.VsENBP1 regulates the expression of the early nodulin PsENOD12B[J].Plant Molecular Biology,1999,40:495-506.
[10]Kereszt A,Li D,Indrasumunar A,et.al.Agrobacterium rhizogenes-mediated transformation of soybean to study root biology[J].Nature Protocols,2007,2(4):948-952.
[11]Cao D,Hou W,Song S,et.al.Assessment of conditions affecting Agrobacterium rhizogenes mediated transformation of soybean[J].Plant Cell Tissue and Organ Culture,2009,96:45-52.
[12]Ane J M,Kiss G B,Riely B K,et.al.Medicago truncatula DMI1 required for bacterial and fungal symbioses in legumes[J].Science,2004,303:1364-1367.
[13]Bersoult A,Camut S,Perhald A,et.al.Expression of the Medicago truncatula DMI2 gene suggests roles of the symbiotic nodulation receptor kinase in nodules and during early nodule development[J].Molecular Plant-Microbe Interactions,2005,18:869-876.
[14]Frendo P,Harrison J,Norman C,et.al.Glutathione and homoglutathione play a critical role in the nodulation process of Medicago truncatula[J].Molecular Plant-Microbe Interactions,2005,18:254-259.
[15]Kaló P,Gleason C,Edwards A,et.al.Nodulation signaling in legumes requires NSP2,a member of the GRAS family of transcriptional regulators[J].Science,2005,308:1786-1789.
[16]Limpens E,Mirabella R,Fedorova E,et.al.Formation of organelle-like N2fixing symbiosomes in legume root nodules is controlled by DMI2[J].Proceedings of the National Academy of Sciences,USA,2005,102:10375-10380.
[17]Vieweg M F,Hohnjec N,Küster H.Two genes encoding different truncated hemoglobins are regulated during root nodule and arbuscular mycorrhiza symbioses of Medicago truncatula[J].Planta,2005,220:757-766.
[18]Cheon C I,Lee N G,Siddique A B,et.al.Roles of plant homologs of Rab1p and Rab7p in the biogenesis of the peribacteroid membrane,a subcellular compartment formed de novo during root nodule symbiosis[J].EMBO Journal,1993,12(11):4125-4135.
[19]Lee N G,Stein B,Suzuki H,et.al.Expression of antisense nodulin-35 RNA in Vigna aconitifolia transgenic root nodules retards peroxisome development and affects nitrogen availability to the plant[J].The Plant Journal,1993,3:599-606.
[20]Taylor C G,Fuchs B,Collier R,et.al.Generation of composite plants using Agrobacterium rhizogenes[J].Methods Molecular Biology,2006,343:155-167.
[21]Hayashi S,Gresshoff P M,Kinkema M.Molecular analysis of lipoxygenases associated with nodule development in soybean[J].Molecular Plant-Microbe Interactions,2008,21:843-853.
[22]Cho H J,Farrand S K,Noel G R,et.al.High-efficiency induction of soybean hairy roots and propagation of the soybean cyst nematode[J].Planta,2000,210:195-204.
?[23]Klink V P,Kim K H,Martins V,et.al.A correlation between host-mediated expression of parasite genes as tandem inverted repeats and abrogation of development of female Heterodera glycines cyst formation during infection of Glycine max[J].Planta,2009,230:53-71.
[24]Li J,Todd T C,Trick H N.Rapid in planta evaluation of root expressed transgenes in chimeric soybean plants[J].Plant Cell Reports,2010,29:113-123.
[25]Kifle S,Shao M,Jung C,et.al.An improved transformation protocol for studying gene expression in hairy roots of sugar beet(Beta vulgaris L.)[J].Plant Cell Reports,1999,18:514-519.
[26]Cai D,Thurau T,Tian Y,et.al.Sporamin-mediated resistance to beet cyst nematodes(Heterodera schachtii Schm.)is dependent on trypsin inhibitory activity in sugar beet(Beta vulgaris L.)hairy roots[J].Plant Molecular Biology,2003,51:839-849.
[27]Remeeus P M,van Bezooijen J,Wijbrandi J,et.al.In vitro testing is a reliable way to screen the temperature sensitivity of resistant tomatoes against Meloidogyne incognita[M]In Proceedings of 5th International Symposium on Crop Protection.Belgium:Universiteit Gent,1998,63:635-640.
[28]Li J R,Todd T C,Oakley T R,et.al.Host-derived suppression of nematode reproductive and fitness genes decreases fecundity of Heterodera glycines Ichinohe[J].Planta,2010,232:775-785.

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Last Update: 2014-08-04