QTL Mapping for 100 seed Weight Using Wild Soybean Chromosome Segment Substitution Lines(PDF)

¡¶´ó¶¹¿Æѧ¡·[ISSN:1000-9841/CN:23-1227/S]

Issue:

2014Äê02ÆÚ

Page:

154-160

Research Field:

Publishing date:

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Title:

QTL Mapping for 100 seed Weight Using Wild Soybean Chromosome Segment Substitution Lines

Author(s):

CHEN Qing-shan¹; JIANG Hong-wei ¹; 2; SUN Dian-jun²; LIU Chun-yan²; XIN Da-wei¹; ZENG Qing-li¹; MA Zhan-zhou¹; HU Guo-hua¹; 2

1.Agronomy College,Northeast Agricultural University,Harbin 150030,China;

2.Land Reclamation Research and Breeding Centre of Heilongjiang,Harbin 150090,China

Keywords:

Soybean; Chromosome segment substitution lines; 100 seed weight; QTL mapping

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2014.02.0154

Abstract:

A chromosome segment substitution lines(BC3)including 130 lines was constructed by the cross of wild soybean ZYD00006(donor parent)and cultivar Suinong 14(recurrent parent).The QTL underlying 100 seed weight was identified using ANOVA Method based on single marker with trait.Twenty five SSR markers underlying 100 seed weight were detected with ANOVA method.To avoid the false positive of co segregation markers,substitution mapping was used to verify the result of ANOVA method.Finally,Nineteen QTL underlying 100 seed weight were identified using two methods and those QTL distributed on 10 linkage groups.Seven QTL were in full accord with known results;two QTL were somewhat different with known results of 0.9 cM or ?4.6 cM ?distance.Another 10 ones were first discovery of loci,which should be specific loci in the study.QSW-D1a2 and QSWH2 with 3.6 and -2.1 of additive effects,fragments length were less than 10 cM could be used as the first choice loci for further study.In this study,substitution lines which had similar genetic background were used to QTL mapping.The result of QTL mapping is more credible because there is no interference of genetic background.Specific materials and important loci lay a foundation for further study on 100ª²seed weight QTL fine mapping and molecular assisted breeding.

References:

[1] Mian M A R,Bailey M A,Tamulonis J P,et al.Molecular markers associated with seed weight in two soybean populations[J].Theoretical and Applied Genetics,1996,93:1011-1016.
[2]Li D,Pfeiffer T W,Cornelius P L.Soybean QTL for yield and yield components associated with alleles[J].Crop Science,2008,48:571-581.
[3]Maughan P J,Maroof M A S,Buss G R.Molecular-marker analysis of seed-weight:genomic locations,gene action,and evidence for orthologous evolution among three legume species[J].Theoretical and Applied Genetics,1996,93:574-579.
[4]Chen Q S,Zhang Z C,Liu C Y,et al.QTL analysis of major agronomic traits in soybean[J].Agricultural Sciences in China,2007,6:399-405.
[5]Sun Y,Pan J,Shi X,et al.Multi-environment mapping and meta-analysis of 100-seed weight in soybean[J].Molecular Biology Reports,2012,39:9435-9443.
[6] Íôϼ , ÐìÓî , Àî¹ã¾ü , µÈ . ´ó¶¹°ÙÁ£ÖØ QTL ¶¨Î» [J]. ×÷Îïѧ±¨ ,2010,36(10):1674-1682.(Wang X,Xu Y,Li G J,et al.Mapping quantitative trait loci for 100-seed weight in soybean(Glycine max L.Merr.)[J].Acta Agronomica Sinica,2010,36(10):1674-1682.)
[7] ÍõÁ¢Çï , ÕÔÓÀ·æ , ѦÑǶ« , µÈ . ÓñÃ×ÏνÓʽµ¥Æ¬¶Îµ¼ÈëϵȺÌåµÄ¹¹½¨ºÍÆÀ¼Û [J]. ×÷Îïѧ±¨ ,2007,33(4):663-668.(Wang L Q,Zhao Y F,Xue Y D,et al.Development and evaluation to two link-up single segment introgression lines(SSILs)of maize(Zea maysL.)[J].Acta Agronomica Sinica,2007,33(4):663-668.)
[8]Gur A,Zamir D.Unused natural variation can lift yield barriers in plant breeding[J].PLoS Biology,2004,2:e245.
[9]Zamir D.Improving plant breeding with exotic genetic libraries[J].Nature Reviews Genetics,2001,2:983-989.
[10]Li M,Sun P,Zhou H,et al.Identification of quantitative trait loci associated with germination using chromosome segment substitution lines of rice(Oryza sativa L.)[J].Theoretical and Applied Genetics,2011,123:411-420.
[11]Koide Y,Fujita D,Tagle A G,et al.QTL for spikelet number from a high yielding rice variety,Hoshiaoba,detected in an introgression line with the genetic background of an indica rice variety,IR64[J].Euphytica,2013,192:97-106.
[12]Qi H,Huang J,Zheng Q,et al.Identification of combining ability loci for five yield related traits in maize using a set of testcrosses with introgression lines[J].Theoretical and Applied Genetics,2013,126:369-377.
[13]Davoyan E R,Davoyan R O,Bebyakina I V,et al.Identification of a leaf-rust resistance gene in species of AegilopsL.,synthetic forms,and introgression lines of common wheat[J].Russian Journal of Genetics:Applied Research,2012,2:325-329.
[14]Lei M P,Li G R,Zhou L,et al.Identification of wheat-Secale africanum chromosome 2Rafr introgression lines with novel disease resistance and agronomic characteristics[J].Euphytica,2013,194:197-205.
[15]Zhang W B,Qiu P C,Jiang H W,et al.Dissection of genetic overlap of drought and low-temperature tolerance QTLs at the germination stage using backcross introgression lines in soybean[J].Molecular Biology Reports,2012,39:6087-6094.
[16]Wang W,He Q,Yang H,et al.Development of a chromosome segment substitution line population with wild soybean(Glycine soja Sieb.et Zucc.)as donor parent[J].Euphytica,2013,189:293-307.
[17] ÔøÇìÁ¦ , ½¯ºéε , Áõ´ºÑà , µÈ . ÀûÓøßÊÀ´ú»Ø½»ÈºÌå¶Ô´ó¶¹Ð¡Á£ÐÔ×´µÄ»ùÒòÐÍ·ÖÎö¼° QTL ¶¨Î» [J]. ÖйúÓÍÁÏ×÷Îïѧ±¨ ,2012,34(5):473-477.(Zeng Q L,Jiang H W,Liu C Y,et al.Genotype analysis and QTL mapping small seed size soybean with advanced back[J] .Chinese Journal of Oil Crop Sciences,2012,34(5):473-477.)
[18] ÇñÀö¾ê . ´ó¶¹ÖÖÖÊ×ÊÔ´ÃèÊö¹æ·¶ºÍÊý¾Ý±ê×¼ [M]. ±±¾© : ÖйúÅ©Òµ³ö°æÉç ,2006.(Qiu L J.Descriptors and date standard for soybean(Glycine max L.Merr.)[M].Beijing:Chinese Agricultural Press,2006.)
[19]Doyle J J.Isolation of plant DNA from fresh tissue[J].Focus,1990,12:13-15.
[20]Fonc¨¦ka D,HodoAbalo T,Rivallan R,et al.Genetic mapping of wild introgressions into cultivated peanut:a way toward enlarging the genetic basis of a recent allotetraploid[J].BMC Plant Biology,2009,9:103.
[21]McCough S R,Doerge R W.QTL mapping in rice[J].Trends in Genetics,1995,11:482-487.
[22] ºÎ·ç»ª , ϯÕÂÓª , ÔøÈðÕä , µÈ . ÀûÓõ¥Æ¬¶Î´ú»»Ïµ¶¨Î»Ë®µ¾³éËëÆÚ QTL[J]. ÖйúÅ©Òµ¿Æѧ ,2005,38(8):1505-1513.(He F H,Xi Z Y,Zeng R Z,et al.Mapping of heading date QTLs in rice(Oryza sativa L.)using single segment substitution lines[J].Scientia Agricultura Sinica,2005,38(8):1505-1513.)
[23]Eshed Y,Zamir D.An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield associated QTL[J].Genetics,1995,141:1147.
[24]Lippman Z B,Semel Y,Zamir D.An integrated view of quantitative trait variation using tomato interspecific introgression lines[J].Current Opinion in Genetics & Development,2007,17:545-552.
[25]Krieger U,Lippman Z B,Zamir D.The flowering gene SINGLE FLOWER TRUSS drives heterosis for yield in tomato[J].Nature Genetics,2010,42:459-463.
[26]Ebitani T,Takeuchi Y,Nonoue Y,et al.Construction and evaluation of chromosome segment substitution lines carrying overlapping chromosome segments of indica rice cultivar ¡®Kasalath¡¯ in a genetic background of japonica elite cultivar ¡®Koshihikari¡¯[J].Breeding Science,2005,55:65-73.
[27]Qi Z M,Sun Y N,Wang J L,et al.Meta-analysis of 100-seed weight QTLs in soybean[J].Agricultural Sciences in China,2011,10:327-334.
[28]Hoeck J A,Fehr W R,Shoemaker R C,et al.Molecular marker analysis of seed size in soybean[J].Crop Science,2003,43:68-74.
[29]Panthee D R,Pantalone V R,West D R,et al.Quantitative trait loci for seed protein and oil concentration,and seed size in soybean[J].Crop Science,2005,45:2015-2022.
[30]Orf J H,Chase K,Jarvik T,et al.Genetics of soybean agronomic traits:I.Comparison of three related recombinant inbred populations[J].Crop Science,1999,39:1642-1651.
[31]Csanadi G Y,Vollmann J,Stift G,et al.Seed quality QTLs identified in a molecular map of early maturing soybean[J].Theoretical and Applied Genetics,2001,103:912-919.
[32]Gong J Y,Wu J R,Wang K,et al.Fine mapping of qHUS6.1,a quantitative trait locus for silicon content in rice(Oryza sativa L.)[J].Chinese Science Bulletin,2010,55:3283-3287.

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