|Table of Contents|

Genetic Diversity of Wild Soybean(Glycine soja)Resistant Germplasms to Soybean Cyst Nematode Revealed by SSR Markers(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年02期
Page:
147-153
Research Field:
Publishing date:

Info

Title:
Genetic Diversity of Wild Soybean(Glycine soja)Resistant Germplasms to Soybean Cyst Nematode Revealed by SSR Markers
Author(s):
YUAN Cui-pingZHAO Hong-kunWANG Yu-minLIU Xiao-dongDONG Ying-shan
Agro Biotechnology Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130033,China
Keywords:
Wild soybeanGenetic diversitySoybean cyst nematodeSSR marker
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.02.0147
Abstract:
Soybean cyst nematode(SCN)(Heterodera glycines Ichinohe)is one of the most worldwide devastating pests in soybean production.Wild soybean(G.soja)is an invaluable gene pool for the genetic improvement of soybean,which probably has genetic variations not present in domesticated soybean(G.max)collection,mining of novel resistant accessions and genes from wild soybean germplasm has become a major objective in soybean research.In the present work,49 SSR markers were selected based on their distribution on the 20 genetic linkage groups of soybean for genetic diversity investigation of 25 G.soja SCN race 3-resistant accessions and for their genetic relationship analysis with 8G.max resistant resources,which were frequently used in breeding program and genetic mapping.High genetic diversity of 25 G.sojaresistant accessions were present with 516 alleles in total and 232 rare alleles at 49 SSR loci.The average effective number of alleles and expected heterozygosity were 6.93 and 0.833 5,respectively.Significant population structure existed among the 33 resistant accessions,6 clusters(C1-C6)were distinguished using a model based Bayesian clustering method in STRUCTURE,all of 8 G.max resistant resources were clustered into C2.The neighbor-joining tree made in POWERMARKER supported the clustering result.The molecular genetic diversity provided vital information for rational utilization of G.soja resistant accessions.

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Last Update: 2014-08-04