|Table of Contents|

Effects of Ascorbic Acid on Soybean Callus Resisting to Osmotic Stress(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2014年01期
Page:
67-69
Research Field:
Publishing date:

Info

Title:
Effects of Ascorbic Acid on Soybean Callus Resisting to Osmotic Stress
Author(s):
CHANG Yun-xia1; XU Ke-dong2; LI Li-li1; ZHOU Lin1; CHEN Long1
1.College of Life Science and Agronomy,Zhoukou Normal University,Zhoukou 466001,China;2.Key Lab of Plant Genetics & Molecular Breeding,Zhoukou Normal University,Zhoukou 466001,China
Keywords:
Ascorbic acid; Osmotic stress; Soybean callus
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.01.0067
Abstract:
The response of soybean callus growth to ascorbic acid (ASA) under salt stress was studied, using soybean Zhongdou 12 as material. Under salt stress, the relative water content(RWC), soluble proteins content(SPC) and increase rate of dry weight(IRDW)were significantly decreased, the contents of proline(Pro) and malondialdehyde (MDA) and the activities of superoxide dismutase (SOD) and peroxidase(POD) were significantly increased(P<0.01). When applied ASA, the RWC, SPC, IRDW, Pro, as well as SOD and POD activities were all increased, while the MDA content was significantly decreased (P<0.01). The results showed that ASA could effectively keep cytomembrane stabilization and reduce the damage from membrane lipid peroxidation, by which could mitigate the harmful effects from salt stress and promote the growth of callus, and the optimal ASA concentration was 8.0 mmol·L-1.

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Last Update: 2014-08-04