Transformation of LePT1 Gene into Soybean via Agrobacterium mediation(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:

2014年01期

Page:

31-34

Research Field:

Publishing date:

Info

Title:

Transformation of LePT1 Gene into Soybean via Agrobacterium mediation

Author(s):

ZHANG Xingzheng ¹ ; WANG Changling ² ; LU Furong ¹ ; HAN Yang ¹ ; SONG Shuhong ²

1.School of Life Sciences,Liaoning University,Shenyang 110036,China;

2.Crop Institute,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China

Keywords:

Soybean; Embryos tips; Transformation;  Agrobacterium mediation;  LePT1

PACS:

S565.1

DOI:

10.11861/j.issn.1000-9841.2014.01.0031

Abstract:

For the sake of cultivating soybean germplasm with higher phosphorus acquisition efficency,a LePT1 gene cloned from tomato genome was transformed into soybean embryos tips via Agrobactium mediation.PCR results showed 1.6 kb band was amplified from T₀ and T₁ genome respectively,moreover,sequencing and online Blast results indicated that the amplified gene was in accordance with LePT1 .In summary,the LePT₁ gene was transformed into T₀ plants and inherited steadily in T₁ generation.

References:

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