Removing Effects of Extract Conditions on Surface Proteins from Soybean Oil Body(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年06期
- Page:
- 835-839
- Research Field:
- Publishing date:
Info
- Title:
- Removing Effects of Extract Conditions on Surface Proteins from Soybean Oil Body
- Author(s):
- ?ZHAO Lu-ping; CHEN Ye-ming; ZHANG Cai-meng; HUA Yu-fei
- ?(School of Food Science and Technology，Jiangnan University，Wuxi 214122，China)
- Keywords:
- Soybean oil body; Oleosin; Soybean proteins; Extract conditions; SDS-PAGE
- PACS:
- -
- DOI:
- 10.11861/j.issn.1000-9841.2013.06.0835
- Abstract:
- ?After confirmed 10% sucrose was beneficial for extracting oil body from raw soybean milk at once ultra centrifugation,the effects of NaCl，urea，EDTA-2Na，SDS and different temperatures on surface proteins of soybean oil body were studied.The variation of oil body surface proteins was characterized by SDSpolyacrylamide gel electrophoresis(SDSPAGE).The results suggested that more than 6 mol·L-1 urea and thermal treatment(80-100℃，15 min)were beneficial for removing soybean proteins which combined with oil body.However，NaCl and EDTA-2Na had no effect on removal of soybean proteins.While SDS was bad for oil body extraction because it could replace oleosins and breake natural structure of oil body.
Last Update: 2014-04-04