Determination of Isoflavones in Extraction of Soybean Dregs Using Ultraviolet Spectrophotometry(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年06期
- Page:
- 818-820
- Research Field:
- Publishing date:
Info
- Title:
- Determination of Isoflavones in Extraction of Soybean Dregs Using Ultraviolet Spectrophotometry
- Author(s):
- ?HE En-ming; SHEN Rui-chi; WANG Wei; CHANG Qiang; GUAN Qi-yang
- ?(Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plant，Fujian Institute of Subtropical Botany，Xiamen 361006，China)
- Keywords:
- Soybean isoflavones; Ultraviolet spectrophotometry; Soybean dregs
- PACS:
- -
- DOI:
- 10.11861/j.issn.1000-9841.2013.06.0818
- Abstract:
- ?A method was established for fast determination of isoflavones in extraction of soybean dregs.Genistein was selected as the reference standard and the samples of extraction of soybean dregs were determined at 260 nm using ultraviolet spectrophotometry.Regression equation was:C=8.052 7A-0.053 2，R2=0.999 5，the linear range was 1.31-11.79 μg·mL-1，and the average recovery was 100.13%，RSD=0.86%.The method has the virtue of simplicity and rapid of operation，high accuracy and good reproducibility，which can be used in production for the rapid determination of isoflavones in extraction of soybean dregs，and provide a reference in soybean isoflavones determination of other samples.
Last Update: 2014-04-04