Study on Screening Concentration of Wide Adaptability Herbicide Resistant? bar Transgenic Soybean(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年06期
- Page:
- 810-813
- Research Field:
- Publishing date:
Info
- Title:
- Study on Screening Concentration of Wide Adaptability Herbicide Resistant? bar Transgenic Soybean
- Author(s):
- ?LI zhi1; 2; WANG Shu-ming1; 2; LIU Jia1; MENG Fan-mei1; YI Zhi-gang1; DONG Zhi-min1; 2
- ?(1.Soybean Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130033,China；2.National Engineering Research Center for Soybean,Changchun 130033,China）
- Keywords:
- bar gene; Transgenic soybean; Herbicide; Glufosinate; Screening
- PACS:
- -
- DOI:
- 10.11861/j.issn.1000-9841.2013.06.0810
- Abstract:
- ?Glufosinate proves to be effective and convenient in screening transgenic soybean plants.To improve the screening efficiency of transgenic positive plants,the glufosinate screening concentrations were determined by two methods(daubing and spraying)in the greenhouse and field,with six common soybean transgenic receptor varieties as materials.The suitable glufosinate screening concentrations for daubing and spraying were 100-150 and 700-750 mg·L-1,respectively,when determined by leaves color changes and mortality rate of soybean plants.Then the availability was tested by T1 plants of soybean transgenic lines with bar gene,the accuracy rate was 61.3% and 73.9%,respectively.The results would facilitate the screening of glufosinate resistant transgenic soybean progenies.
Last Update: 2014-04-04