Effects of Salinization Resistance Transgenic Soybeans on Rhizosphere Soil Nitrogen-fixing Bacterial Diversity(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年06期
- Page:
- 801-805
- Research Field:
- Publishing date:
Info
- Title:
- Effects of Salinization Resistance Transgenic Soybeans on Rhizosphere Soil Nitrogen-fixing Bacterial Diversity
- Author(s):
- ?ZHOU Jie; YU Song; WANG Shan-shan; JIAO Yan-dong; LIU Zhi-hua; WANG Hong-yan
- ?(Resources and Environment Sciences College,Northeast Agricultural University,Harbin 150030,China)
- Keywords:
- Salinization resistance; Transgenic soybeans; nifH; Diversity; DGGE
- PACS:
- -
- DOI:
- 10.11861/j.issn.1000-9841.2013.06.0801
- Abstract:
- ?The molecular diversity of soil bacteria nitrogenase nifH gene after planting the salt tolerance of transgenic soybean,(SRTS),its recipient parent Heinong 35,as well as Hefeng 50,Kangxianwang and Yesheng 21 was analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCRDGGE),so as to provide technical basis for establishing soil ecological security assessment system for transgenic crops in saline soil.The Shannon-Wiener diversity indexes(Dsh)and evenness indexes(Jsh)of SRTS were higher than Heinong 35 without significant difference,while significantly higher than Kangxianwang and Yesheng 21.Cluster analysis of DGGE bands showed SRTS had higher similarity with Heinong 35 and Hefeng 50.Results suggest planting genetically modified soybeans has no obvious influence on diversity of soil nitrogenfixing bacteria.
Last Update: 2014-04-04