Interaction between Fomesafen and Vegetable Protein(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年06期
- Page:
- 763-767
- Research Field:
- Publishing date:
Info
- Title:
- Interaction between Fomesafen and Vegetable Protein
- Author(s):
- -
- ?(1.College of Agriculture，Northeast Agricultural University，Harbin 150030，China;2.College of Food Science ＆ Nutritional Engineering，China Agricultural University，Beijing 100083，China)
- Keywords:
- Fomesafen; Bovine serum albumin(BSA); Vegetable protein; Fluorescence; Thermodynamic parameter
- PACS:
- -
- DOI:
- 10.11861/j.issn.1000-9841.2013.06.0763
- Abstract:
- ?Taking the bovine serum albumin(BSA) as control,the interaction between fomesafen and vegetable protein was studied by fluorescence spectrum under the imitated physiological condition of animal body.Changes of the binding constant and the number of binding site of the fomesafen with BSA and vegetable protein after the protein denaturalization reaction were analyzed. Fomesafen had a strong ability to quench the fluorescence launching from BSA and vegetable protein.The BSA and vegetable protein had reacted with fomesafen and they formed a certain new compound，and its belonged to static fluorescence queching reaction.The thermodynamic parameters obtained at different temperature suggested that the binding power between the BSA and fomesafen was mainly the hydrophobic interaction，between the vegetable protein and fomsafen was mainly hydrogen bonding/van der Waals.When the three proteins were subjected to heat，ultrasonic，metal salts/sonic and acidity treatment，their binding reaction with fomesafen varied to some extent.This essay will supply a theory base for the poisoning mechanism of fomesafen.
Last Update: 2014-02-28