Isolation and Expression Patterns of GsRNF12 Gene in Glycine soja(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年06期
- Page:
- 750-754
- Research Field:
- Publishing date:
Info
- Title:
- Isolation and Expression Patterns of GsRNF12 Gene in Glycine soja
- Author(s):
- -
- ?（1.College of Life Science and Technology,Heilongjiang Bayi Agricultural University，Daqing 163319,China；2.Daqing Bureau of Quality and Technical Supervision Standardization,Daqing 163319,China；3.Institute of Botany,Chinese Academy of Sciences，Beijing 100093,China；4.College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing 163319,China）
- Keywords:
- Glycine soja; GsRNF12 gene; Gene expression; Stress
- PACS:
- -
- DOI:
- 10.11861/j.issn.1000-9841.2013.06.0750
- Abstract:
- ?The GsRNF12 gene from Glycine soja was amplified by RTPCR.Its full length cDNA I was 723 bp and contained a open reading frame（ORF）,encoding a polypeptide of 240 amino acid.GsRNF12 protein in 178220 amino acid has a typical C4HC3 domain structure,belonging to the C3HC4 zinc finger protein family.The expression patterns of GsRNF12 gene were analyzed using quantitative realtime PCR.The results indicated GsRNF12 gene responded differently to salt,cold,drought,ABA and SA；GsRNF12 had no tissuespecific expression characteristics which expressed in roots,stems and leaves,and the expression in root was higher than that in other tissues.
Last Update: 2014-02-28