Cloning of Conserved Sequence of HC-Pro Gene of Soybean Mosaic Virus and Construction of Its RNAi Vector(PDF)
《大豆科学》[ISSN:1000-9841/CN:23-1227/S]
- Issue:
- 2013年06期
- Page:
- 744-749
- Research Field:
- Publishing date:
Info
- Title:
- Cloning of Conserved Sequence of HC-Pro Gene of Soybean Mosaic Virus and Construction of Its RNAi Vector
- Author(s):
- -
- ?(National Key Laboratory for Crop Genetics and Germplasm Enhancement/National Center for Soybean Improvement/Key Laboratory of Biology and Genetic Improvement of Soybean,Ministry of Agriculture,P.R.China,Nanjing Agricultural University,Nanjing 210095,China)
- Keywords:
- Soybean mosaic virus; HC-Pro gene; Cloning; RNAi; GATEWAY technology; Vector construction
- PACS:
- -
- DOI:
- 10.11861/j.issn.1000-9841.2013.06.0744
- Abstract:
- ?Soybean mosaic virus(SMV）is one of the major viral diseases in soybean,which severely affects the soybean production and quality.RNA interference(RNAi)can specifically silence the homologous target genes in mRNA level,which provides a new strategy for virusresistant crop breeding.In this study,we made the nucleotide sequence alignment of HCPro genes from 11 popular strains of SMV in the world and cloned 268 bp the most conserved gene fragment using the cDNA of SC3 strain.Using the GATEWAY technology,the RNAi vector pB7GWIWG2(II)HC-Proi suitable for agrobacterium mediated transformation of soybean was constructed.Identification of the sequence and the direction of HC-Proi in the vector through the primers of 35S promoter and terminator showed that the recombination of HC-Pro and pB7GWIWG2(II)formed the inverted repeat.It may lay the foundation for improvement of soybean resistance to SMV using RNA interference technology.
Last Update: 2014-02-28